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SRX20678506: GSM7476434: Intramuscular adipocytes, Ad_ lncFABP4 transduction, Day4, rep2 [Ad_ lncFABP4-2]; Bubalus bubalis; RNA-Seq
1 DNBSEQ (DNBSEQ-G400) run: 24.1M spots, 7.2G bases, 4.3Gb downloads

External Id: GSM7476434_r1
Submitted by: Guangxi university
Study: Effect of overexpress of lncFABP4 on gene expression during adipogenic differentiation of buffalo intramuscular adipocytes
show Abstracthide Abstract
To investigate the function lncFABP4 in the regulation of adipogenic differentiation, we overexpressed lncFABP4 in buffalo intramuscular adipocytes Overall design: First, intramuscular adipocytes were planted in six-well plates in triplicate. When the cells reach 80% confluence, transduction was performed. Adenovirus were added to cells at the indicated MOI. Then, The culture medium was exchanged three hours later. Next, When transduction was performed for 48 hours, cells were treated with an inducing medium. Finally, Cells were collected for RNA sequencing after 48 hours of induction.
Sample: Intramuscular adipocytes, Ad_ lncFABP4 transduction, Day4, rep2 [Ad_ lncFABP4-2]
SAMN35734107 • SRS17973566 • All experiments • All runs
Organism: Bubalus bubalis
Library:
Name: GSM7476434
Instrument: DNBSEQ-G400
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNAs were isolated using a TRIzol regent according to a manufacturer's protocol (Invitrogen, Carlsbad, CA, USA). Take a certain amount of total RNA samples, and use oligo dT beads to enrich mRNA with poly A tail; mRNA molecules were fragmented into small pieces; The fragmented mRNA was synthesized into first strand cDNA using random primers; The second strand cDNA was synthesized; The synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments; PCR amplification and purification; Library quality control; Library circularization; The library was amplified to make DNA nanoball (DNB); Sequencing on DNBSEQ (DNBSEQ Technology) platform.
Runs: 1 run, 24.1M spots, 7.2G bases, 4.3Gb
Run# of Spots# of BasesSizePublished
SRR2491760824,064,2857.2G4.3Gb2024-06-14

ID:
28108169

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