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SRX20726094: Short read illumina sequencing of Acinetobacter spp
1 ILLUMINA (Illumina HiSeq 2000) run: 372,211 spots, 177.1M bases, 62.2Mb downloads

Design: Pure cultures of each strain are grown in plates or broth. Cells are harvested and resuspended in a tube with cryoperservative (MicrobankTM, Pro-Lab Diagnostics UK, United Kingdom) or with DNA/RNA Shield (Zymo Research, USA) following MicrobesNG strain submission procedures. Strain containing tubes are sent to MicrobesNG for sequencing.Five to forty microlitres of the suspension are lysed with 120 L of TE buffer containing lysozyme (final concentration 0.1 mg/mL) and RNase A (ITW Reagents, Barcelona, Spain) (final concentration 0.1 mg/mL), incubated for 25 min at 37C. Proteinase K (VWR Chemicals, Ohio, USA) (final concentration 0.1mg/mL) and SDS (Sigma-Aldrich, Missouri, USA) (final concentration 0.5% v/v) are added and incubated for 5 min at 65C. Genomic DNA is purified using an equal volume of SPRI beads and resuspended in EB buffer (Qiagen, Germany).DNA is quantified with the Quant-iT dsDNA HS kit (ThermoFisher Scientific) assay in an Eppendorf AF2200 plate reader (Eppendorf UK Ltd, United Kingdom).
Submitted by: University of Birmingham
Study: Comparison of Acinetobacter baumannii and Acinetobacter lwoffii
PRJNA918592 • SRP415943 • All experiments • All runs
show Abstracthide Abstract
Comparing the whole genome sequences of A. baumannii and A. lwoffii with respect to antibiotic resistance.
Sample: AL28 WT2 Gen
SAMN35800885 • SRS18016777 • All experiments • All runs
Organism: Acinetobacter lwoffii
Library:
Name: 252342
Instrument: Illumina HiSeq 2000
Strategy: WGS
Source: GENOMIC
Selection: unspecified
Layout: PAIRED
Runs: 1 run, 372,211 spots, 177.1M bases, 62.2Mb
Run# of Spots# of BasesSizePublished
SRR24968178372,211177.1M62.2Mb2024-03-19

ID:
28156514

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