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SRX21540295: replicate = biological replicate 3 for BAE_0 16S rDNA
1 ILLUMINA (Illumina MiSeq) run: 145,209 spots, 87.4M bases, 46.4Mb downloads

Design: For each sample, total DNA from fresh soil (0.5 g) was extracted using an E.Z.N.A. Soil DNA kit (Omega, USA). The quantity and purity of DNA were determined using NanoDrop2000 microspectrophotometry (Thermo Fisher Scientific, USA). The V3-V4 region of bacterial 16S rRNA was amplified using the 341F (5- CCTAYGGGRBGCASCAG-3) and 806R (5- GGACTACNNGGGTATCTAAT-3) primers. The samples were sequenced on an Illumina MiSeq PE356 platform (Majorbio, China) in a paired-end manner.
Submitted by: Hubei Academy of Agricultural Sciences
Study: Paddy Soil microbial Community during Degradation of Rice Straw
show Abstracthide Abstract
The degradation of rice straw in paddy soils was simulated using a 28-day pot experiment. We investigated the effects of bioaugmentation and biostimulation by introducing a lignocellulolytic microbial consortium and supplying the available nutrients. The V3-V4 region (341F/806R) of bacterial 16S rRNA was amplified and sequenced to analysis the soil microbial community during this process.
Sample: replicate = biological replicate 3 for BAE_0 16S rDNA
SAMN37191820 • SRS18749709 • All experiments • All runs
Library:
Name: BAE_0c
Instrument: Illumina MiSeq
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 145,209 spots, 87.4M bases, 46.4Mb
Run# of Spots# of BasesSizePublished
SRR25818153145,20987.4M46.4Mb2023-08-30

ID:
29023937

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