replicate = biological replicate 2 for BAE_21 16S rDNA - SRA

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SRX21540307: replicate = biological replicate 2 for BAE_21 16S rDNA
1 ILLUMINA (Illumina MiSeq) run: 69,007 spots, 41.5M bases, 22.6Mb downloads

Design: For each sample, total DNA from fresh soil (0.5 g) was extracted using an E.Z.N.A. Soil DNA kit (Omega, USA). The quantity and purity of DNA were determined using NanoDrop2000 microspectrophotometry (Thermo Fisher Scientific, USA). The V3-V4 region of bacterial 16S rRNA was amplified using the 341F (5- CCTAYGGGRBGCASCAG-3) and 806R (5- GGACTACNNGGGTATCTAAT-3) primers. The samples were sequenced on an Illumina MiSeq PE367 platform (Majorbio, China) in a paired-end manner.

Submitted by: Hubei Academy of Agricultural Sciences

Study: Paddy Soil microbial Community during Degradation of Rice Straw

PRJNA1010947 • SRP457615 • All experiments • All runs

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The degradation of rice straw in paddy soils was simulated using a 28-day pot experiment. We investigated the effects of bioaugmentation and biostimulation by introducing a lignocellulolytic microbial consortium and supplying the available nutrients. The V3-V4 region (341F/806R) of bacterial 16S rRNA was amplified and sequenced to analysis the soil microbial community during this process.

Sample: replicate = biological replicate 2 for BAE_21 16S rDNA

SAMN37191831 • SRS18749721 • All experiments • All runs

Organism: straw metagenome

Library:

Name: BAE_21b

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 69,007 spots, 41.5M bases, 22.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR25818142	69,007	41.5M	22.6Mb	2023-08-30

ID:: 29023949

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