WGS of evolved Bacillus subtilis : P4bC21 - SRA

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SRX22112506: WGS of evolved Bacillus subtilis : P4bC21
1 ILLUMINA (NextSeq 550) run: 1.6M spots, 491.2M bases, 164.1Mb downloads

Design: Paired-end (evolution alone) or single-end (evolution with Pseudomonas) libraries were prepared using the NEBNext Ultra II DNA Library Prep with Sample Purification Beads. Fragment reads were generated on an Illumina NextSeq sequencer using TG NextSeq 500/550 High Output Kit v2 (300 cycles) (RNOmique Platform, UdeS). The methodology for processing the reads drew inspiration from the GenPipes framework. We first implemented a quality-based trim of the reads using fastp 0.21.0, setting the parameters to --cut_right --cut_window_size 4 --cut_mean_quality 30 --length_required 30.

Submitted by: Universite de Sherbrooke

Study: Bacillus subtilis laboratory evolution on tomato roots

PRJNA1028130 • SRP466740 • All experiments • All runs

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The goal was to determine if B. subtilis can adapt to the inhibitory effect of Pseudomonas on roots. Two adaptative laboratory evolution experiments were performed with B. subtilis in mono-association or co-cultured with P. fluorescens on tomato plant roots. Evolved isolates with various colony morphology and stronger colonization capacity of both tomato plant and A. thaliana roots emerged rapidly from the two evolution experiments. Evolved isolates were sequenced at different cycles of evolution.

Sample:

SAMN37813257 • SRS19176900 • All experiments • All runs

Organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10

Library:

Name: P4bC21

Instrument: NextSeq 550

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 1.6M spots, 491.2M bases, 164.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR26406919	1,626,411	491.2M	164.1Mb	2023-12-21

ID:: 30044996

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