RNA-Seq of Thunnus maccoyii: sexuyally immature male and female gonad... - SRA

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SRX1074516: RNA-Seq of Thunnus maccoyii: sexuyally immature male and female gonads: Sample SBT_OC6
1 ILLUMINA (Illumina HiSeq 2500) run: 30M spots, 6G bases, 3.8Gb downloads

Design: "Five biological replicates (originating from different animals) of Thunnus maccoyii ovaries were collected. Ovary sample from each fish was further dissociated to obtain enriched oogonia cell suspensions, by using a 30um size filtration. The enriched cell suspension was homogenized and total RNA was then extracted using RNAZol RT according to the manufacturer's instructions. Total RNA was sent on dry ice to the Australian Genome Research Facility (AGRF; Brisbane, QLD) for library preparation and RNA-Seq on an Illumina HiSeq2500 platform, producing 100 base paired end reads. All five replicates from each treatment were multiplexed on each lane. The Illumina CASAVA 1.8.2 pipeline was used to generate the sequence data."

Submitted by: University of the Sunshine Coast

Study: RNA-Seq of Southern bluefin tuna (Thunnus maccoyii) gonad cells

PRJNA288188 • SRP059929 • All experiments • All runs

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RNA-Seq analysis of Southern bluefin tuna (Thunnus maccoyii) male and female gonad cells, to identify expression patterns of germ cell and gonadal-specific genes. RNA-Seq data produced by Illumina HiSeq 2500 platform, using the CASAVA pipeline version 1.8.2.

Sample: Southern bluefin tuna size-filtered ovary cells from fish SBT_6

SAMN03796059 • SRS973224 • All experiments • All runs

Organism: Thunnus maccoyii

Library:

Name: SBT_OC6_C5NARACXX_ACAGTG_L004

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 30M spots, 6G bases, 3.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR2079766	29,968,026	6G	3.8Gb	2016-03-15

ID:: 1560291

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