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SRX22587324: OLC2703_Escherichia coli
1 ILLUMINA (Illumina MiSeq) run: 1.9M spots, 937.6M bases, 589.2Mb downloads

Design: Brain Heart Infusion broth was inoculated with a single colony of bacteria and incubated for 18-24 h at 35C. Genomic DNA was isolated from 400 L of culture using the Maxwell 16 Cell SEV DNA Purification kit (Promega, Madison, WI). DNA was then quantified using the Quant-iT High-Sensitivity DNA Assay Kit (Life Technologies Inc., Burlington, ON). Sequencing libraries were constructed from 1 ng of gDNA using Nextera XT DNA Sample Preparation and Index Kits (Illumina, Inc., San Diego, CA) according to manufacturers instructions. Genomic sequencing was performed on the Illumina MiSeq Platform (Illumina, Inc.) using a 600-cycle MiSeq Reagent kit v3 (Illumina, Inc.).
Submitted by: Canadian Food Inspection Agency
Study: Colicin-producing E. coli
PRJNA1038773 • SRP473064 • All experiments • All runs
show Abstracthide Abstract
Colicin-producing E. coli isolated from foods, shown to inhibit growth of Shigella sonnei
Sample: OLC2703_Escherichia coli
SAMN38336047 • SRS19594241 • All experiments • All runs
Organism: Escherichia coli
Library:
Name: 2017-SEQ-0984
Instrument: Illumina MiSeq
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 1.9M spots, 937.6M bases, 589.2Mb
Run# of Spots# of BasesSizePublished
SRR268930321,948,563937.6M589.2Mb2023-11-20

ID:
30587176

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