RAD-Seq of Rana muscosa: SJ population - SRA

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SRX23094842: RAD-Seq of Rana muscosa: SJ population
1 ILLUMINA (Illumina NovaSeq 6000) run: 2.9M spots, 873.4M bases, 294.6Mb downloads

Design: MboI (GATC/CTAG) and PstI (CTGCAG/GACGTC) were used for library preparation. DNA samples were digested for 4 hours with MboI/PstI and indexes were ligated for 8 hours at 16C. An initial 15 cycle PCR reaction was performed to add a biotinylated primer and second index to the pools, and samples were purified on streptavidin beads. Final libraries were made by running 4 cycles of PCR on eluted DNA. Individual libraries were pooled at equimolar ratios, precipitated and size selected (280-500 bp) on Pippin Prep.

Submitted by: San Diego Zoo Wildlife Alliance

Study: Mountain yellow legged-frog RADseq data

PRJNA1061082 • SRP481346 • All experiments • All runs

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To provide improved breeding recommendations for Rana muscosa and conduct molecular population genetic assessments, double digest restriction site-associated DNA sequencing data was generated from 78 frogs coming from 3 populations, two of them managed ex situ.

Sample: MYLF

SAMN39254686 • SRS20051073 • All experiments • All runs

Organism: Rana muscosa

Library:

Name: 908136_S275

Instrument: Illumina NovaSeq 6000

Strategy: RAD-Seq

Source: GENOMIC

Selection: Restriction Digest

Layout: PAIRED

Runs: 1 run, 2.9M spots, 873.4M bases, 294.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR27420298	2,911,473	873.4M	294.6Mb	2024-02-09

ID:: 31202337

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