RNA_seq_ of_lobster_larva_53 - SRA

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SRX23949610: RNA_seq_ of_lobster_larva_53
1 ILLUMINA (Illumina NovaSeq 6000) run: 21.7M spots, 6.5G bases, 2Gb downloads

Design: RNA extraction was performed according to Novogenes internal protocols, which include an automated homogenization method followed by a filtration column. Sample integrity and quantity were determined using an Agilent Bioanalyzer 2100 and sample purity was checked using a Nanodrop. Libraries were prepared using poly-A enrichment to select for mRNA following Novogenes internal quality control assays. Sequencing was done using the Illumina NovaSeq platform. Samples were sequenced as paired-end reads of 150 base pairs, and those reads in the fastq format were processed for downstream analyses or cleaned by removing reads containing adaptors, reads with >10% uncertain nucleotides, and reads with >50% low quality nucleotides, defined as Base Quality < 5. Reads were aligned to a reference sequence for American lobster (Polinski et al., 2021) using HISAT2 (version 2.0.5) and assembled into transcripts using StringTie (version 1.3.3b). Raw counts of map reads were aggregated using featureCounts (version 1.5.0-p3)

Submitted by: University of New England

Study: Gene expression in larval lobster, Homarus americanus

PRJNA1087720 • SRP495220 • All experiments • All runs

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We are characterizing the gene expression during the development of stages I through V in the American lobster, Homarus americanus.

Sample: Larvae 53

SAMN40453502 • SRS20751283 • All experiments • All runs

Organism: Homarus americanus

Library:

Name: k18ZL4G3_1

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Runs: 1 run, 21.7M spots, 6.5G bases, 2Gb

Run	# of Spots	# of Bases	Size	Published
SRR28343019	21,686,290	6.5G	2Gb	2024-03-14

ID:: 32254899

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