Instrument: Illumina Genome Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Queens sampled were flash frozen with dry ice and were immediately transfer to -80°C until further analyses. Eviscerated abdomens with attached fat bodies from individual bees were dissected under dry ice. Total RNA samples were extracted from the abdominal fat bodies using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. RNA quantity and quality were assayed with a ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington DE) and a 2100 Bioanalyzer (Agilent, Santa Clara, CA). RNA libraries were prepared for sequencing using standard Illumina protocols