RNA-seq of Zea Mays: kernel LOX4oe event 2 treated - SRA

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SRX24297323: RNA-seq of Zea Mays: kernel LOX4oe event 2 treated
1 ILLUMINA (Illumina NovaSeq 6000) run: 22.9M spots, 6.9G bases, 2.2Gb downloads

Design: Total RNA was isolated using TRI Reagent (Sigma-Aldrich, St. Louis, MO, USA) and purified with the RNA Clean up protocol (Qiagen, Valencia, CA, USA), according to the manufacturer's instructions (Lanubile et al., 2013). The extracted RNA was quantified using a fluorometric assay (Qubit, Thermo Fisher Scientific Inc. Waltham, MA, USA) and the integrity was checked using the 4150 TapeStation System (Agilent, Santa Clara, CA, USA) as well as gel electrophoresis.cDNA libraries (three lines x two conditions x three biological replicates) were constructed following the manufacturer's instructions of the Universal Plus mRNA-Seq kit (Tecan Genomics, Redwood City, CA, USA) and checked with a fluorometric assay (Qubit, Thermo Fisher Scientific Inc.). Libraries were sequenced on paired-end 150 bp mode on NovaSeq 6000 platform (Illumina, San Diego, CA, USA) at IGA-Tech (Udine, Italy)as service provider.

Submitted by: Catholic University of Sacred Heart

Study: Overexpression of a lipoxygenase gene for improving pathogen resistance in maize

PRJNA1101865 • SRP502539 • All experiments • All runs

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The transgenic overexpression of lipoxigenase 4 gene (ZmLOX4) was carried out to investigate the possible implication of this candidate gene in the resistance mechanisms against Fusarium verticillioides

Sample: Z321Fv_rep1

SAMN41003938 • SRS21061474 • All experiments • All runs

Organism: Zea mays

Library:

Name: ID3044_22-Z321Fv-P1-F03

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 22.9M spots, 6.9G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR28731221	22,927,616	6.9G	2.2Gb	2024-04-18

ID:: 32609983

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