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DRX527240: Illumina HiSeq 4000 paired end sequencing of SAMD00765544
1 ILLUMINA (Illumina HiSeq 4000) run: 348,078 spots, 70.3M bases, 34.6Mb downloads

Submitted by: Plant Genetics and Breeding, Graduate School of Environmental and Life Science, Okayama University (Plant Genetics and Breeding, Graduate School of En)
Study: Genotyping-by-sequencing of cucumber genetic resources
PRJDB14335 • DRP011736 • All experiments • All runs
show Abstracthide Abstract
This project aimed to select the World Cucumber Core Collection based on genetic variation and population structure chracterized by genotyping-by-sequencing for cucumber genetic resources deposited in the National Agriculture and Food Research Organization (NARO) Genebank, Japan.
Sample: 457
SAMD00765544 • DRS393970 • All experiments • All runs
Organism: Cucumis sativus
Library:
Instrument: Illumina HiSeq 4000
Strategy: OTHER
Source: GENOMIC
Selection: Reduced Representation
Layout: PAIRED
Construction protocol: Modified genotyping-by-sequencing (Elshire et al. 2011) strategy, using Hsp92II or NlaIII restriction enzyme to digest genomic DNA, followed by ligation of custom sequencing adapters including sample-specific barcodes, PCR amplification, and pooling of libraries from 192 samples into the same sequencing run.
Pipeline: show...hide...
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1NIL
Runs: 1 run, 348,078 spots, 70.3M bases, 34.6Mb
Run# of Spots# of BasesSizePublished
DRR543459348,07870.3M34.6Mb2024-07-01

ID:
33515674

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