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DRX043906: Illumina HiSeq 2500 paired end sequencing of SAMD00042726
1 ILLUMINA (Illumina HiSeq 2500) run: 21.1M spots, 4.3G bases, 2.5Gb downloads

Submitted by: NIBB
Study: RNA-seq analysis of alligator gonad during sex determination
show Abstracthide Abstract
The American alligator (Alligator mississippiensis) displays temperature-dependent sex determination (TSD), in which incubation temperature during embryonic development determines the sexual fate of the individual. Alligator sex is determined during stage 21-24, and the gonad is bipotential prior to sex determination. In this study, A. mississippiensis embryos were incubated under female-producing temperature (30C) until embryonic stage 19, at which point the eggs were split into two incubating temperatures, male- (33.5C) and female-producing temperature, and sampled over the course of 36 days. This study was conducted to analyze the influence of initial environmental temperature upon gene expression and also extensively profile gonad-specific gene expression pattern that occurs during TSD by using RNA-seq analysis.
Sample: Day 6 gonad MPT 3
SAMD00042726 • DRS021750 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol:
Spot descriptor:
forward102  reverse

Runs: 1 run, 21.1M spots, 4.3G bases, 2.5Gb
Run# of Spots# of BasesSizePublished
DRR04862321,054,1334.3G2.5Gb2016-03-09

ID:
2318029

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