Genome sequencing of Mediterraneibacter gnavus Strain RI1 - SRA

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SRX25432326: Genome sequencing of Mediterraneibacter gnavus Strain RI1
1 OXFORD_NANOPORE (GridION) run: 142,445 spots, 2.1G bases, 1.7Gb downloads

Design: In this study, we isolated M. gnavus strain RI1 from healthy Japanese human feces. The species of the isolates were determined by a BLAST search of the 16S rRNA gene sequence, which showed 99% identity with M. gnavus ATCC 29149. Cultivation of M. gnavus strain RI1 was performed overnight at 37C in an anaerobic chamber. Bacteria genomic DNA extracted using a NucleoBond high molecular weight (HMW) DNA kit (Macherey-Nagel). The sequencing library for long reads was prepared using a rapid barcoding kit (SQK-RBK114; Oxford Nanopore Technologies), sequenced in a flow cell (FLO-MIN114) with a GridION device (Oxford Nanopore Technologies), and base called (guppy 6.5.7, super accurate mode), demultiplexed and adapter trimmed with a GridION X5 version 23.07.12 software (Oxford Nanopore Technologies).

Submitted by: Keio University

Study: Mediterraneibacter gnavus Genome sequencing and assembly

PRJNA1139382 • SRP521844 • All experiments • All runs

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We report the complete genome sequence of Mediterraneibacter gnavus strain RI1, a Gram-positive anaerobic gut microbe isolated from human feces. The complete circular genome has a genome size of 3.25 Mb, with a G+C content of 42.6%.

Sample: Microbe sample from Mediterraneibacter gnavus

SAMN42772178 • SRS22092325 • All experiments • All runs

Organism: Mediterraneibacter gnavus

Library:

Name: Mediterraneibacter gnavus Strain RI1

Instrument: GridION

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 142,445 spots, 2.1G bases, 1.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR29938738	142,445	2.1G	1.7Gb	2024-07-24

ID:: 34102160

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