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Submitted by: Brandie Nelson
Study: Org.one project - Jacutinga (Aburria/Pipile jacutinga) - Sequencing with ONT P2 Solo
show Abstracthide Abstract
Rapid sequencing of the critically endangered Jacutinga as part of the ORG.one project https://org.one/oo. Samples were opportunistic blood samples stored in DNA Shield and extracted with the QIAGEN MagAttract HMW DNA Kit. Library prep was completed with the SQK-LSK114 Ligation Kit from Oxford Nanopore Technologies (ONT) and loaded into an R10.4.1 Flow Cell for the PromethION P2 Solo. The P2 interfaced with ONT MinKNOW software to capture the raw reads in various pod5 files.
Library:
Name: Aburriajacutinga_library
Instrument: PromethION
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Experiment attributes: (show all 9 attributes...) (hide...)
basecaller version: dorado 7.3.11
Oxford Nanopore flowcell type: R10.4.1
Oxford Nanopore instrument: PromethION
Oxford Nanopore instrument latitude: -93.18497
Oxford Nanopore instrument location (region and locality): St. Paul, MN
Oxford Nanopore instrument longitude: 44.98138
Oxford Nanopore kit type: LSK-114
read N50 (kbp): 18.3
total (Gbp): 64.1
Run# of Spots# of BasesSizePublished
ERR13509916unavailable2024-08-24

ID:
34861982

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