short-read data of Aedes aegypti - SRA

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SRX26026527: short-read data of Aedes aegypti
1 ILLUMINA (Illumina HiSeq 4000) run: 1.7M spots, 487.3M bases, 211.9Mb downloads

Design: DNA extraction from carcasses using Chemagic360 or Qiagen Dneasy kit. PCR-free library prep protocol. At least 150ng of gDNA from each sample were fragmented to target size between 350 and 400 basepairs. libraries were pooled and sequenced on the Illumina HiSeq 4000 and HiSeqX platforms to generate 2x150 basepair reads. Reads mapped to the Ae. aegypti L5 genome reference (NCBI WGS Project NIGP01) using BWA mem (v0.7.16) with default parameters, then sorted using samtools sort (1.1), and duplicate reads were marked using Picards markdup function (2.1.0, http://broadinstitute.github.io/picard/). Unmapped reads and their pairs are included in this file.

Submitted by: Bucknell University

Study: EVE-X: Software to Identify Novel Viral Insertions in Wild-Caught Arthropod Hosts from Next-Generation Short Read Data

PRJNA1158798 • SRP531586 • All experiments • All runs

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Aedes aegypti sequence data from "EVE-X: Software to Identify Novel Viral Insertions in Wild-Caught Arthropod Hosts from Next-Generation Short Read Data", Molecular Ecology Resources, 2024

Sample:

SAMN43548808 • SRS22598347 • All experiments • All runs

Organism: Aedes aegypti

Library:

Name: Australia_20

Instrument: Illumina HiSeq 4000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 1.7M spots, 487.3M bases, 211.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR30603924	1,655,486	487.3M	211.9Mb	2024-10-04

ID:: 35131210

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