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SRX2980161: SRA.total.ox.hi5
1 ILLUMINA (NextSeq 500) run: 52.9M spots, 1.4G bases, 943.7Mb downloads

Design: Hi5 cells were cultured in Express Five serum free medium and then total RNAs were isolated from collected Hi5 cells using mirVana miRNA isolation kit (Ambion). We size selected 18-40nt long small RNAs and followed protocol "Zamore Lab Illumina TrueSeq Small RNA Cloning protocol (April, 2014)" to generate oxidized small RNA library. Oxidized library only contains small RNAs with modification (in this case 2′-O-methylation) at the 3′ ends.
Submitted by: University of Massachusetts Medical School
Study: Trichoplusia ni isolate:ovarian cell line Hi5 Genome sequencing and assembly
show Abstracthide Abstract
We assembled the Trichoplus ni genome to establish the animal and the ovarian cell line Hi5 as a new model for studying small RNAs.
Sample: SRA.total.ox.hi5
SAMN07312343 • SRS2333379 • All experiments • All runs
Organism: Trichoplusia ni
Library:
Name: SRA.total.ox.hi5
Instrument: NextSeq 500
Strategy: OTHER
Source: OTHER
Selection: size fractionation
Layout: SINGLE
Runs: 1 run, 52.9M spots, 1.4G bases, 943.7Mb
Run# of Spots# of BasesSizePublished
SRR580069352,871,5441.4G943.7Mb2017-12-31

ID:
4247447

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