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SRX3478093: GSM2891759: B200; Lynx rufus; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 25.9M spots, 2.6G bases, 1.4Gb downloads

Submitted by: NCBI (GEO)
Study: Genome-wide expression reveals multiple systemic effects of anticoagulant poisons in bobcats (Lynx rufus)
PRJNA422754 • SRP126924 • All experiments • All runs
show Abstracthide Abstract
We evaluated AR-related effects on genome wide expression patterns in a population of bobcats in southern California. We identify differential expression of genes involved in xenobiotic metabolism, endoplasmic reticulum stress response, epithelial integrity, and both adaptive and innate immune function. Further, we find that differential expression of immune related genes may be attributable to AR-related effects on leukocyte differentiation. Collectively, our results provide an unprecedented understanding of the sublethal effects of AR exposure on a wild carnivore. Overall design: Blood samples from 26 bobcats tested positive for Anticoagulant Rodenticides (Ars) and 26 samples of bobcats not tested positive for ARs.
Sample: B200
SAMN08196027 • SRS2764080 • All experiments • All runs
Organism: Lynx rufus
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted from 0.5 mL whole blood using the Ambion Mouse RiboPure Blood extraction kit, followed by globin removal using the Ambion GlobinClear Mouse kit (Life Technologies, Inc). RNA was quantified on the Agilent bioanalyzer (Agilent Technologies, USA). Each sample was uniquely tagged with custom index sequences developed at UCLA (Faircloth et al., 2014) comparable to Illumina TruSeq tags. Individual sample libraries were then pooled in equimolar ratios, with 13 or 14 samples per pool and each pool sequenced on two lanes of an Illumina HiSeq 2500 or HiSeq 4000 sequencer
Experiment attributes:
GEO Accession: GSM2891759
Links:
Runs: 2 runs, 25.9M spots, 2.6G bases, 1.4Gb
Run# of Spots# of BasesSizePublished
SRR638440912,892,6731.3G707Mb2018-03-26
SRR638441012,985,2751.3G715.2Mb2018-03-26

ID:
4841240

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