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SRX3526722: GSM2909517: Bacillus subtilis; T49; Bacillus subtilis; RNA-Seq
1 ION_TORRENT (Ion Torrent Proton) run: 11.7M spots, 1.2G bases, 901.8Mb downloads

Submitted by: NCBI (GEO)
Study: Analysis of differential gene expression during Bacillus subtilis spore outgrowth
show Abstracthide Abstract
The transcriptome of 8 strains were studied during 7 stages of the Morphological phases of sporulation P0 = Resuspention of cells in sporulation medium; P1 = Onset of sporulation before asymmetric division; P2 = Visible asymmetric septum; P3 = Ongoing engulfment of the forespore compartment by the mother cell compartment of sporulating cells; P4 = Completed engulfment (engulfed phase-dark forespores are surrounded by the mother-cell cytoplasm); P5 = Maturation (ongoing dehydration of the forespore core seen as a transition of phase-dark forespores into phase-bright foresppores); P6 = Phase-bright forespores (sporulation almost completed, mother-cells contain phase-bright dehydrated forespores). Overall design: 8 strains 7 time points
Sample: Bacillus subtilis; T49
SAMN08284969 • SRS2802445 • All experiments • All runs
Library:
Instrument: Ion Torrent Proton
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Cell were lysed using glassbead and totRNA was isolated using phenol/chloroform and ethanol precipitation Standard library preparation kit for IonProton
Experiment attributes:
GEO Accession: GSM2909517
Links:
Runs: 1 run, 11.7M spots, 1.2G bases, 901.8Mb
Run# of Spots# of BasesSizePublished
SRR643516611,742,8271.2G901.8Mb2018-03-09

ID:
4901649

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