RNA-seq of Northoprocta perdicaria: adult male gonad - SRA

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SRX3686951: RNA-seq of Northoprocta perdicaria: adult male gonad
1 ILLUMINA (NextSeq 500) run: 38.2M spots, 5.7G bases, 2.4Gb downloads

Design: Flash frozen tissues stored in RNAlater at -80 C were homogenized with a Tissumizer (Tekmar). Total RNA was extracted from each specimen using the RNeasy mini kit (Qiagen). RNA samples were processed with a PrepX PolyA kit (Wafergen Biosystems) on the Apollo 324 (IntegenX) to enrich for polyadenylated transcripts. Stranded RNA-seq libraries were generated from these samples using the PrepX mRNA 48 kit (Wafergen Biosystems) on the Apollo 324.

Submitted by: Harvard University

Study: Transcriptome sequencing for genome annotation in palaeognaths

PRJNA433114 • SRP132742 • All experiments • All runs

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Transcriptome sequencing from multiple tissues from emu (Dromaius novaehollandiae) and Chilean tinamou (Nothoprocta perdicaria) to aid in genome annotation.

Sample:

SAMN08476480 • SRS2946967 • All experiments • All runs

Organism: Nothoprocta perdicaria

Library:

Name: ctin_M_gonad

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Runs: 1 run, 38.2M spots, 5.7G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR6713507	38,244,484	5.7G	2.4Gb	2018-02-12

ID:: 5092912

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