Leaves of Talinum - SRA

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SRX3745127: Leaves of Talinum
1 ILLUMINA (NextSeq 500) run: 26.7M spots, 7.8G bases, 3.6Gb downloads

Design: Library preparation was performed at Genotypic Technology s Genomics facility following Illumina TruSeq RNA library protocol outlined in TruSeq RNA Sample Preparation Guide (Part # 15008136;Rev. A; Nov 2010). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNAwas fragmented for 2 minutes at elevated temperature (94 o C) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers.Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNAwas cleaned up using Agencourt Ampure XP SPRI beads (Beckman Coulter). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments. The prepared library was quantified using Nanodrop and validated for quality by running ana liquot on High Sensitivity Bioanalyzer Chip (Agilent).

Submitted by: CSIR-Central Food Technological Research Institute (CFTRI)

Study: Transcriptome of Talinum fruticosum leaves

PRJNA436076 • SRP133549 • All experiments • All runs

show Abstracthide Abstract

Talinum grown widely in Asia and the leaves of Talinum are edible. It was therefore decided to do the transcriptome of the leaves to elucidate the nutritional value of the same. This is the first report of Talinum transcriptome.

Sample: Leaves of Talinum

SAMN08612657 • SRS3001502 • All experiments • All runs

Organism: Talinum fruticosum

Library:

Name: Talinum

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 26.7M spots, 7.8G bases, 3.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR6785589	26,710,027	7.8G	3.6Gb	2018-02-27

ID:: 5163779

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