Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted from 1.0 mL whole blood preserved in 2.5 mL PAXGene blood RNA solution using the PAXGene Blood RNA extraction kit, followed by globin removal using the Ambion GLOBINClear Mouse kit (Life Technologies, Inc). RNA was quantified on the Agilent Bioanalyzer (Agilent Technologies, USA). cDNA libraries were created using a TruSeq Library Prep Kit v2 (Illumina, Madison, WI) and quantified with the KAPA SYBR® Fast qPCR library quantification kit (Kapa Biosystems Inc., Wilmington, MA). Individual sample libraries were pooled in equimolar ratios with 8 to 10 samples per pool. Each pool was sequenced on a single lane of an Illumina HiSeq 2000 or HiSeq 4000 sequencing platform.