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ERX2603485: Illumina HiSeq 2000 paired end sequencing; RNA-seq across tissues in four Notothenioid species (Antarctic icefish)
1 ILLUMINA (Illumina HiSeq 2000) run: 18.1M spots, 3.7G bases, 2.1Gb downloads

Design: RNA-seq across tissues in four Notothenioid species (Antarctic icefish)
Submitted by: Institut de biologie de l’Ecole normale superieure (IBENS), Ecole normale superieure, CNRS, INSERM, PSL Universite Paris 75005 Paris, France (Institut de biologie de l’Ecole normale superieu)
Study: RNA-seq across tissues in four Notothenioid species (Antarctic icefish)
PRJEB26835 • ERP108862 • All experiments • All runs
show Abstracthide Abstract
We sequenced and assembled de novo the coding transcriptomes in four species of Notothenioid fish: Neopagetopsis ionah (Jonah's ice fish), Pseudochaenichtys georgianus (South Georgia icefish), Harpagifer antarcticus (Antarctic spiny plunderfish) and Parachaenichthys charcoti (Charcot's dragonfish). We sampled 1-4 individuals and 1-14 tissues (brain, white muscle, liver, head kidney, trunk kidney, skin, heart, red muscle, spleen, ovary, testis, whole blood, gill, red blood cells) in each species, depending on tissue availability.
Sample: Sample 51
SAMEA4669945 • ERS2490558 • All experiments • All runs
Organism: Pseudochaenichthys georgianus
Library:
Name: Sample 51_p
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Construction protocol: Samples were flash-frozen in liquid nitrogen upon dissection of wild-caught specimens. Total RNA was extracted using TRIsure (Bioline) according to manufacturer's instructions and purified on RNeasy columns (Qiagen). Libraries were prepared using Illumina TruSeq RNA Library Prep Kit according to manufacturer's instructions.
Spot descriptor:
1  forward102  reverse

Experiment attributes:
Experimental Factor: organism: Pseudochaenichthys georgianus
Experimental Factor: organism part: ventricle
Experimental Factor: cell type: not applicable
Runs: 1 run, 18.1M spots, 3.7G bases, 2.1Gb
Run# of Spots# of BasesSizePublished
ERR258720918,100,5093.7G2.1Gb2018-07-03

ID:
5854854

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