Induced_sample - SRA

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SRX4381582: Induced_sample
1 ILLUMINA (Illumina HiSeq 2000) run: 67.4M spots, 10.1G bases, 6.2Gb downloads

Design: The mRNA was isolated from the total RNA of T. kiharae induced sample using RNA purification beads followed by fragmentation and priming for cDNA synthesis as recommended by the manufacturer (Illumina, USA). For double-stranded cDNA synthesis, the SuperScript Double-Stranded cDNA Synthesis kit (Invitrogen, USA) was used, further purification was accomplished using Agencourt AMPure XP beads (Beckman Coulter, Inc, USA). End repairing and 3-end adenylation were performed following the RNA adapter ligation. Upon enrichment of DNA fragments library templates were validated using Agilent 2100 Bioanalyzer (Agilent, USA). Clonal clusters were created from DNA library templates using TruSeq PE Cluster Kit v2 and cBot automated system (Illumina, USA). Clusters obtained were used to carry out paired-end runs by Genome Analyzer IIx (Illumina, USA). Illumina HiSeq2000 sequencing was carried out on the equipment of EIMB RAS Genome Center.

Submitted by: Vavilov Institute of General Genetics

Study: Triticum kiharae cultivar:Dorof. et Migush. Raw sequence reads

PRJNA479515 • SRP153095 • All experiments • All runs

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We have performed RNA sequencing and de novo transcriptome assembly to explore the diversity of defensin-like (DEFL) genes in the wheat Triticum kiharae and to study their role in induced resistance (IR) mediated by elicitor metabolites of a non-pathogenic strain FS-94 of Fusarium sambucinum.

Sample:

SAMN09643004 • SRS3537804 • All experiments • All runs

Organism: Triticum kiharae

Library:

Name: Induced

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 67.4M spots, 10.1G bases, 6.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR7511483	67,443,211	10.1G	6.2Gb	2018-11-27

ID:: 5946500

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