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SRX915090: GSM1630296: brain MR26 27weeks rotenone; Nothobranchius furzeri; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 40.1M spots, 2G bases, 1.3Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: RNA-seq of the killifish N.furzeri during normal aging (cross-sectional and longitudinal study) and treatment with rotenone
show Abstracthide Abstract
The study consists of three parts: 1) normal aging in liver and skin (cross-sectional); 2) treatment with rotenone in brain, liver and skin; 3) longitudinal study of 45 fish with different ages at their death measured at two different time points by fin clipping Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de) Overall design: 185 samples: 1) 50 normal aging samples: 2 tissues (liver and skin) * 5 age groups (5,12,20,27,39 weeks) * 5 replicates; 2) 45 samples: 3 tissues (brain, liver and skin) * 2 age groups (9,27 weeks) for controls and 1 age group for treatment group * 5 replicates; 3) 90 samples: 45 fish, each measured at 10 and 20 weeks (fin clipping)
Sample: brain MR26 27weeks rotenone
SAMN03396494 • SRS870113 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: For library preparation an amount of around 2.5 μg of total RNA per sample was processed using Illumina’s TruSeqTM RNA Sample Prep Kit (Illumina; San Diego; CA, USA) following the manufacturer’s instruction. Total RNA was isolated using QIAzol (Qiagen, Hilden, Germany) based on the phenol/chloroform extraction method. Afterwards the RNA was quantified photometrically with a NanoDrop 1000 (PeqLab, Erlangen, Germany) and stored at -80 °C until use.
Experiment attributes:
GEO Accession: GSM1630296
Links:
External link:
Runs: 1 run, 40.1M spots, 2G bases, 1.3Gb
Run# of Spots# of BasesSizePublished
SRR187340040,111,9512G1.3Gb2015-12-03

ID:
1314034

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