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SRX1118858: GSM1832976: A. duranensis methylC-seq; Arachis duranensis; Bisulfite-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 343.1M spots, 38.1G bases, 14.8Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: The genomes of the ancestral species of peanut - a remarkable case of living archaeology
show Abstracthide Abstract
Peanut (Arachis hypogaea) has a large (~2.7 Gbp) allotetraploid genome with closely related component genomes making its genome very challenging to assemble. Here we report genome sequences of its diploid ancestors (A. duranensis and A. ipaënsis). We show they are similar to the peanut's A- and B-genomes and use them use them to identify candidate disease resistance genes, create improved tetraploid transcript assemblies, and show genetic exchange between peanut's component genomes. Based on remarkably high DNA identity and biogeography, we conclude that A. ipaënsis may be a descendant of the very same population that contributed the B-genome to cultivated peanut. Overall design: Whole Genome Bisulphite Sequencing of the peanut species Arachis duranensis and Arachis ipaensis.
Sample: A. duranensis methylC-seq
SAMN03938701 • SRS1011551 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: Bisulfite-Seq
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Construction protocol: Leaves were ground in liquid nitrogen and DNA extracted using QIAGEN DNAeasy Plant Mini Kit (Cat. No. 69106). Libraries were constructed according to Urich et. al. MethylC-seq library preparation for base-resolution whole-genome bisulfite sequencing. Nature Protocols 10, 475–483 (2015).
Experiment attributes:
GEO Accession: GSM1832976
Links:
Runs: 2 runs, 343.1M spots, 38.1G bases, 14.8Gb
Run# of Spots# of BasesSizePublished
SRR2128064204,805,64320.7G8.9Gb2016-01-04
SRR2128065138,275,83617.4G6Gb2016-01-04

ID:
1632789

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