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SRX1740298: GSM2142284: Xylem-2; Populus trichocarpa; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 14.5M spots, 1.4G bases, 935.3Mb downloads

Submitted by: NCBI (GEO)
Study: RNA-seq of major tissues and xylem cell types of Populus trichocarpa
show Abstracthide Abstract
We used the stem cross-sections of P. trichocarpa and our recently developed laser capture microdissection (LCM) to collect fibers, vessels, and a combination of 3 cell types (fibers + vessels + rays). Total RNA from the three samples was isolated, amplified, and analyzed by full-transcriptome RNA-sequencing. The sequencing reads were mapped to the P. trichocarpa genome. Overall design: Different major tissues (shoot tip, leaf, phloem, primary root, stem differentiating xylem) and xylem tissue cell types (fiber, vessel, ray cells) were analyzed by RNA-seq.
Sample: Xylem-2
SAMN04939134 • SRS1420047 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was isolated using RNeasy Plant RNA isolation kit (Qiagen), and RNAs of LCM samples were amplified using Arcrurus RiboAmp HS PLUS kit (Life Technology). RNA libraries were prepared for sequencing using standard Illumina protocols.
Experiment attributes:
GEO Accession: GSM2142284
Links:
Runs: 1 run, 14.5M spots, 1.4G bases, 935.3Mb
Run# of Spots# of BasesSizePublished
SRR347300414,465,0791.4G935.3Mb2017-01-19

ID:
2496104

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