Whole genome sequencing of Campylobacter sp. strains - SRA

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SRX16772145: Whole genome sequencing of Campylobacter sp. strains
1 ILLUMINA (Illumina MiSeq) run: 652,039 spots, 158.2M bases, 90.5Mb downloads

Design: Illumina libraries were prepared for all strains using the KAPA Low-Throughput Library Preparation Kit with Standard PCR Amplification Module (Kapa Biosystems, Wilmington, MA), following manufacturer's instructions except for the following changes: 750 ng DNA was sheared at 30 psi for 40 s and size selected to 700-770 bp following Illumina protocols. Standard desalting TruSeq LT and PCR Primers were ordered from Integrated DNA Technologies (Coralville, IA) and used at 0.375 and 0.5 M final concentrations, respectively. PCR was reduced to 3-5 cycles. Libraries were quantified using the KAPA Library Quantification Kit (Kapa), except with 10 l volume and 90-s annealing/extension PCR, then pooled and normalized to 4 nM. Pooled libraries were requantified by ddPCR on a QX200 system (Bio-Rad), using the Illumina TruSeq ddPCR Library Quantification Kit and following manufacturer's protocols, except with an extended 2-min annealing/extension time. The libraries were sequenced 2 250 bp paired end v2 on a MiSeq instrument (Illumina) at 13.5 pM, following manufacturer's protocols.

Submitted by: USDA, ARS, WRRC

Study: Campylobacter sp. RM12920 Genome sequencing and assembly

PRJNA667455 • SRP389921 • All experiments • All runs

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Campylobacter species from cattle and swine in California

Sample:

SAMN14886870 • SRS14398511 • All experiments • All runs

Organism: Campylobacter sp. RM12920

Library:

Name: RM12920WGS_MiSeq

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 652,039 spots, 158.2M bases, 90.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR20751902	652,039	158.2M	90.5Mb	2022-08-10

ID:: 23530892

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