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SRX21356867: Camelina sativa isolate:Joelle
1 PACBIO_SMRT (Sequel II) run: 1.9M spots, 28.6G bases, 20.7Gb downloads

Design: DNA was extracted from etiolated shoot tips from a single individual for each of the two genotypes of camelina using a standard CTAB extraction protocol with the exception that the mixing and extraction steps were performed with no vigorous shaking. DNA shearing was performed using the Diagenode Megaruptor 2 (Diagenode Inc., Denville, NJ, USA) with the 20 Kb fragment protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA, USA). The library was size-selected using a size-limiting solid phase reversible immobilization-bead cleanup to remove library molecules smaller than 3 Kb in length to generate the final libraries for sequencing. Sequencing was performed on a Sequel II System using Binding Kit 2.0, Sequencing Kit 2.0, and SMRT Cell 8M (Pacific Biosciences, Menlo Park, CA, USA). The library was sequenced using a 30-hour movie time on 2 SMRT cells for each library. Raw subreads were converted to HiFi data using the circular consensus sequencing process to call a single high-quality consensus sequence for each molecule with a consensus accuracy cutoff of 99.5%.
Submitted by: USDA-ARS-ETSARC
Study: Camelina sativa isolate:Joelle | cultivar:Joelle Genome sequencing
PRJNA995623 • SRP454918 • All experiments • All runs
show Abstracthide Abstract
Camelina sativa is an emerging oilseed crop. Its genome has previously been sequenced using short read technologies. Here we report an improved assembly of a winter-type variety (Joelle) based on PacBio Hifi sequencing.
Sample:
SAMN36496493 • SRS18601388 • All experiments • All runs
Organism: Camelina sativa
Library:
Name: Joelle_PacBio_HiFi
Instrument: Sequel II
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 1.9M spots, 28.6G bases, 20.7Gb
Run# of Spots# of BasesSizePublished
SRR256300521,867,04928.6G20.7Gb2023-08-14

ID:
28794519

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