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SRX10105754: Shotgun DNA sequencing of Enterococcus faecium
1 ILLUMINA (Illumina MiSeq) run: 972,885 spots, 248.7M bases, 125.9Mb downloads

Design: DNA extracted using DNeasy Blood and Tissue Kit (Qiagen) from a single bacterial colony, libraries prepared using Nextera XT DNA sample preparation kit (Illumina)
Submitted by: Copenhagen University Hospital Hvidovre
Study: Introduction and clonal spread of vanB Enterococcus faecium in Copenhagen, Denmark, and development of a rapid and accurate clone specific vanB E. faecium PCR
PRJNA702038 • SRP306536 • All experiments • All runs
show Abstracthide Abstract
In 2018-2019, an increase of vanB vancomycin-resistant Enterococcus faecium (VREfm) was observed in the Capital Region of Denmark. vanA/vanB PCR is accurate in detection of vanA; however, the positive predictive value for vanB+ samples are low, because of the presence of vanB in non-enterococcal gut commensals. The aims of the study were to investigate the epidemiology and clonal relatedness of vanB VREfm from 2015-2019, and to develop a clone-specific vanB VREfm PCR for rapid and accurate detection of vanB VREfm in rectal screening samples.
Sample:
SAMN17922646 • SRS8262674 • All experiments • All runs
Organism: Enterococcus faecium
Library:
Name: 18379
Instrument: Illumina MiSeq
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 972,885 spots, 248.7M bases, 125.9Mb
Run# of Spots# of BasesSizePublished
SRR13717334972,885248.7M125.9Mb2021-02-16

ID:
13233330

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