Whole Genome Sequencing of Bacillus subtilis - SRA

SRX21193934: Whole Genome Sequencing of Bacillus subtilis
1 ILLUMINA (Illumina HiSeq 4000) run: 6.6M spots, 2.1G bases, 675.4Mb downloads

Design: The DNA fragments undergo end repair where in the mix converts the overhangs resulting from fragmentation into blunt ends. The 3 to 5 exonuclease activity of end repair mix removes the 3 overhangs and polymerase activity fills in the 5 overhangs. To the blunt ended fragments adenylation is performed by adding single A nucleotide to the 3 ends. To the adenylated fragments loop adapters were ligated and cleaved with uracil-specific excision reagent (USER) enzyme. The samples are further purified using AMPure beads and The DNA is then enriched by PCR with 6 cycles using NEBNext Ultra II Q5 master mix, illumina universal primer and sample-specific octamer primers.

Submitted by: BIT Mesra Ranchi-835215

Study: Bacillus subtilis strain:RNC-6 Genome sequencing

PRJNA1000251 • SRP452230 • All experiments • All runs

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Characterization and genome analysis of Bacillus subtilis

Sample:

SAMN36765789 • SRS18452927 • All experiments • All runs

Organism: Bacillus subtilis

Library:

Name: RNC-6

Instrument: Illumina HiSeq 4000

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 6.6M spots, 2.1G bases, 675.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR25461129	6,597,393	2.1G	675.4Mb	2023-07-31

ID:: 28629963

SRA

Sequence Read Archive

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