WGS of Legionella from healthcare facilities - SRA

SRX4475785: WGS of Legionella from healthcare facilities
1 ILLUMINA (Illumina MiSeq) run: 1.4M spots, 546.4M bases, 317.1Mb downloads

Design: Frozen stocks of Legionella were cultured on buffered-charcoal yeast-extract (BCYE) agar and incubated at 37C to obtain isolated colonies for DNA extraction. Nucleic acids from both clinical and environmental samples of Legionella were extracted for WGS with a modified Masterpure DNA Isolation Kit procedure (Epicentre, Madison, WI, USA) . Extracted DNAs were diluted to a final concentration of 2ng/_L before WGS submission.

Submitted by: Wadsworth Center

Study: Long-term persistence of Legionella in healthcare facilities

PRJNA483184 • SRP155508 • All experiments • All runs

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We investigated the value of whole-genome sequencing (WGS) and single nucleotide polymorphism (SNP) analyses in determining the relationships among and evolutionary rates of Legionella species with long-term persistence in three healthcare facilities. We examined retrospective clinical and environmental isolates of Legionella micdadei and Legionella pneumophila serogroup 1 isolates with identical PFGE DNA fingerprints sampled over the course of up to 18 years.

Sample: NH-1

SAMN09727263 • SRS3600895 • All experiments • All runs

Organism: Legionella pneumophila

Library:

Name: NH-1

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 1.4M spots, 546.4M bases, 317.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR7610972	1,350,767	546.4M	317.1Mb	2018-07-27

ID:: 6061676

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