WGS of 15 divergently selected maize lines for stalk strength - SRA

SRX10469716: WGS of 15 divergently selected maize lines for stalk strength
1 ILLUMINA (Illumina HiSeq 1000) run: 599.3M spots, 45.5G bases, 17.8Gb downloads

Design: Individual leaf samples from 96 plants of each of the three populations (C0, C15-L, and C15-H) were collected from field-grown plants at the V8 stage, flash-frozen in liquid nitrogen, and stored at -80C. DNA of each plant was extracted as published (Saghai-Maroof et al. 1984), cleaned using the Qiagen DNeasy 96 Plant kit (Qiagen, Germantown, MD), diluted to 20ng/l, and approximately 200ng DNA was used for modified ApeKI genotyping-by-sequencing (GBS) (Elshire et al. 2011). The modification included separating each 96 well plate into 4 pools of 24 adapter-ligated samples. All 288 individually barcoded samples were pooled and single-end 75-bp sequencing was performed on NextSeq High Output sequencer at University of Missouri, Columbia.

Submitted by: University of Missouri

Study: Genetic Architecture of Maize Rind Strength Revealed by the Analysis of Divergently Selected Populations

PRJNA718320 • SRP312628 • All experiments • All runs

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The strength of the stalk rind, measured as rind penetrometer resistance (RPR), is an important contributor to stalk lodging resistance. To enhance the genetic architecture of RPR, we combined selection mapping on populations developed by 15 cycles of divergent selection for high and low RPR with time-course transcriptomic and metabolic analyses of the stalks.

Sample: Plant sample from Zea mays subsp. mays

SAMN18492123 • SRS8599865 • All experiments • All runs

Organism: Zea mays subsp. mays

Library:

Name: HKJNYBGX3

Instrument: Illumina HiSeq 1000

Strategy: WGS

Source: GENOMIC

Selection: Restriction Digest

Layout: SINGLE

Runs: 1 run, 599.3M spots, 45.5G bases, 17.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR14097307	599,265,931	45.5G	17.8Gb	2021-03-30

ID:: 13883476

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