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SRX9122728: RNA-Seq of homo sapiens
1 ILLUMINA (NextSeq 500) run: 29.6M spots, 4.5G bases, 1.7Gb downloads

Design: 500 ng of the total RNA was used to create RNA-seq libraries using TruSeq stranded mRNA library preparation kit. Libraries were checked for quality using an Agilent 2100 Bioanalyzer and quantified with DeNovix fluorometer. The sequence was subjected to cluster generation and paired-end 2x75 nucleotide read sequencing on an Illumina NextSeq 500 sequencer and quality check of the raw data files using FASTQC software.
Submitted by: Mahidol University, Faculty of Tropical Medicine
Study: Next Generation Sequencing Analysis of Gene Profiles of Healthy, Hyperlipidaemia, Familail Hyperlipidemia, Coronary Heart Disease and Familial Coronary Heart Disease
show Abstracthide Abstract
To explore genes indicating a risk of familial (premature) coronary heart disease (FH-CHD) development in familial hypercholesterolaemia (FH), 30 Thai male volunteers were enrolled: 7 healthy controls (N), 6 patients with hypercholesterolaemia (H), 4 with FH, 10 with CHD, and 3 with FH-CHD. Transcriptome data were investigated using next-generation sequencing analysis in whole blood (n = 3). Genes that were significantly expressed in both FH and FH-CHD, but not in N, H, and CHD groups, were selected, functionally analysed, and validated by quantitative real-time PCR (RT-qPCR). We identified 55 intersecting genes between FH and FH-CHD groups. The expressions of intersecting genes that shared between FH and FH-CHD groups are potential for further development as predictive markers for FH-CHD in FH patients.
Sample: Hyperlipidemia_3
SAMN16132263 • SRS7365218 • All experiments • All runs
Organism: Homo sapiens
Name: H30029
Instrument: NextSeq 500
Strategy: RNA-Seq
Selection: Oligo-dT
Layout: PAIRED
Runs: 1 run, 29.6M spots, 4.5G bases, 1.7Gb
Run# of Spots# of BasesSizePublished


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