U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Finding primers specific to your PCR template (using Primer3 and BLAST).
PCR Template
(A refseq record is preferred) Help
Help
From To
 
Primer Parameters
Help
Help
Help
Exon/intron selection
Help
Help

Help
Help
Note: Parameter values that differ from the default are highlighted in yellow
Primer Pair Specificity Checking Parameters
Help
Help
GPIPE/139649/100/GCF_002237135.1_top_level
Primer must have at least total mismatches to unintended targets, including
at least mismatches within the last bps at the 3' end. Help
Ignore targets that have or more mismatches to the primer. Help
Help
Help
Help
Note: Parameter values that differ from the default are highlighted in yellow