1R4W,1R4W


Conserved Protein Domain Family
DsbA_GSTK
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cd03021: DsbA_GSTK 
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DsbA family, Glutathione (GSH) S-transferase Kappa (GSTK) subfamily; GSTK is a member of the GST family of enzymes which catalyzes the transfer of the thiol of GSH to electrophilic substrates. It is specifically located in the mitochondria and peroxisomes, unlike other members of the canonical GST family, which are mainly cytosolic. The biological substrates of GSTK are not yet known. It is presumed to have a protective role during respiration when large amounts of reactive oxygen species are generated. GSTK has the same general fold as DsbA, consisting of a thioredoxin domain interrupted by an alpha-helical domain and its biological unit is a homodimer. GSTK is closely related to the bacterial enzyme, 2-hydroxychromene-2-carboxylate (HCCA) isomerase. It shows little sequence similarity to the other members of the GST family.
Links
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Statistics
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PSSM-Id: 239319
Aligned: 15 rows
Threshold Bit Score: 265.755
Created: 10-Feb-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:GSH binding site [chemical binding site]
Evidence:
  • Structure:1R4W; Rattus norvegicus mitochondrial GSTK bound with GSH; contacts at 3.5A
    - View structure with Cn3D
  • Comment:Residues from both subunits in the functional dimer participate in interactions with each GSH molecule.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                  ###                                            #         #            
1R4W_A         6 RVLELFYDVLSPYSWLGFEVLCRYqh----------lWNIKLKLRPALLAGIMKDSGNQ-PPAMVPHKGQYILKEIPLLK 74  Norway rat
EAA56412       4 GKIQCFLDIASYFSYIAFADLLPNldl-------laaHGVEVEFVPVLIAGINTQSGNK-PPWTLQAKAEYLRYDSDRAK 75  Magnaporthe gri...
XP_328075      5 PKITLYLDTVSPFAYEAIKQATNRschistfqndpifKNVDIKYVPIFLGGLMNKCSNT-PPIKIKNKDKWINVERLRWA 83  Neurospora crassa
XP_416525      4 VLVELFYDVVSPYSWLGFEVLCRYqh----------iWNIDLRFRPAFLGGIMQSTGNK-PPAMLPKRAEYLLKDIKRMA 72  chicken
Q09652         5 PRIDFYFDVISPYSYIAFEVFQKLet---------qwKGVTIRYIPFFLGAVMKESGNR-PPAMLPARSIMMMTDLKRTA 74  nematode
Q18973         5 KVVKFFFDVISPYSYFGFEGITRHrs----------vWKTPIQMKPFFFAGVVRHTENPgLPLRIPIKEKYMHKDLLFSA 74  nematode
NP_001002560   6 KVVELFYDVVSPYSWLAFEVLCRYrn----------vWNIDLKLKPAFLGAVMHDSGNR-PPGMIPNKFLYMTSDLKHVS 74  zebrafish
EAA59114       4 PKLTLYYDIVSPFAWIAFKILRDSrt----------fSKCDITYVPVLLGGILKATGNT-PPIRIKNKDKWINQERIRWA 72  Aspergillus nid...
EAA77822       3 GRIDCYLDIVSFYSYTGFIELRRSmse-------laaHNVQVNFIPVFLGGIMSESGNK-PPWVLPAKSRYLAKDSRRSA 74  Gibberella zeae...
EAL90000       4 PKITLYFDVVSPFAHIAFHVLKHSpt----------fAKCDITYVPIFLGGLLKACGST-PPIQIKNKAQWINQERRRWA 72  Aspergillus fum...

Feature 1                                                                                        
1R4W_A        75 q-LFQVPMSVPKDFfgehvkkGTVNAMRFLTAVSMEQPE-------MLEKVSRELWMRIWs-rDEDITESqn-iLSAAEK 144 Norway rat
EAA56412      76 erVGKAGIAFPKDFl-----sMALTVLRAMHYIKRNYSTkqy--ltAMHYLFHAFWTPPHknlTKQDVLRevlaSVPSDF 148 Magnaporthe gri...
XP_328075     84 h-AFSVPIVTDMPPnf---ppNTLPVQRVLAGIEASSSQsqsaviaALDALYKAFWALGQp-iYEPAQLR----SVLASS 154 Neurospora crassa
XP_416525     73 k-YYQVPVTLSEDVfqrilgnSSLGAMRFITAADMTQPK-------YLEPLSREFWLRFWs-qHEDISQVes-iLAVAEK 142 chicken
Q09652        75 k-FWDIPLTPPPLFmewikkyRTTGAMKVLLVLQEQDKE-------LMLRAAREMWVRLWs-rSEKIFEDq--dFVEVLK 143 nematode
Q18973        75 q-YWGIPFRLPKDYtnmmlntSSIVPQRILVASQLRDNV-------LMEDVARGLWHRFYa-yGKPIFTK----SQVAEV 141 nematode
NP_001002560  75 e-YFGVPVRQPSNVfetmfekGSLKAMRFVTAVAEKEKEgd----vKLERVSRELWNRIWs-nDQDITLPas-fAEAGLK 147 zebrafish
EAA59114      73 r-FFSIPIAETSPEgf---ppNTVTVQRALTAISQQSPNkl---ipATEAVWRDFWVKGDgkaTQPEVFV----PIFEKI 141 Aspergillus nid...
EAA77822      75 e-RLAAPYQGSPPDlla--iaKTISPLRALHYIKENYPEgtf--laAMRFLFHKIWLPPHvnlAEDANLIaa-lKEATDK 148 Gibberella zeae...
EAL90000      73 r-YFSVPIVEKTPPgf---paLTVAPQRALCAISKKAPErf---vpAVEALFQSYWVEGNgqiGQPEGFG----PVLEKV 141 Aspergillus fum...

Feature 1                                                   ###                # ###             
1R4W_A       145 AGma-------taQAQHLLNKISTelvKSKLRETTGAACKYGAFGLPTTVAHVd--GKTYMLFGSDRMe----LLAYLLG 211 Norway rat
EAA56412     149 PGqgapl--fssqEVDDIIKAAGTqeyKDALTSSTDEAVAKGAFGAPWIFVTNa-rGAEEPFFGSDRFn----HIYKFLD 221 Magnaporthe gri...
XP_328075    155 LGge--------eAADKVLGAAQTaevKQRLVENTDKAFAEGAFGLPWFTCTNt-kGETEGFWGVDHLg----QVVQFLA 221 Neurospora crassa
XP_416525    143 AGls-------seLTQKLLEMISSpavKNRLKETTEEAVKYGAFGMPAVVAHVn--GKPHLFFGSDRLe----LLASIIG 209 chicken
Q09652       144 AVgv--------kNPEQIVEKSKDekyIKILMENTNKGVDLMAYGAPWINVHTe-dGSEHSFFGSDRFh----LIADLLQ 210 nematode
Q18973       142 LRdlh------vkDVDELVMMSDSaevKNILRENTDEAIGNGCFGAPWMHITDghgKVLQTVFGSDRLp----QVADFLA 211 nematode
NP_001002560 148 AGlt-------asEVDELLTLATSqpiKDKLKSVTSEAIKYKCFGFPTIVCHVd--GKPEIFFGSDRFe----VMAHIIG 214 zebrafish
EAA59114     142 LGs---------sQTKAIMEAANGpeiKTLLGANTQQALDSGAFGLPWFECTNs-rGEKEGFWGIDHLg----QVVEFLG 207 Aspergillus nid...
EAA77822     149 LDggsdsklfsdeDIEKIMKGRESm--KERVKEVTGFALKKGAFGAPWLIATNa-gGKSEAFFGSDRFlgvpfQDVQILP 225 Gibberella zeae...
EAL90000     142 LGk---------dSTEQILKAMSQyeaKSLLLANTDRAFKSGAFGLPWFECTNp-kGETEGFWGIDHLg----QVAEFLG 207 Aspergillus fum...

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