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5GM6: Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution

PDB ID:

5GM6 Download

MMDB ID:

171215

PDB Deposition Date:

2016/7/12

Updated in MMDB:

2022/11

Experimental Method:

electron microscopy

Resolution:

3.5 Å

Source Organism:

Saccharomyces cerevisiae S288C▼

Taxonomy	Molecule
Saccharomyces cerevisiae S288C	A, B, C, F, H, I, J, K, L, N, M, O, P, Q, R, S, T, U, V, W, Y, Z, a, b, c, G, d, X, v, e, f, o, p, q , r, t, n, k, i, h, j, l, m, g
Saccharomyces cerevisiae	D, E

Similar Structures:

Biological Unit for 5GM6: 46-meric; determined by author

Molecular Components in 5GM6
Label	Count	Molecule
Proteins (41 molecules)
A	1	Pre-mrna-splicing Factor 8
B	1	Pre-mrna-splicing Helicase Brr2
C	1	Pre-mrna-splicing Factor Snu114
F	1	Pre-mrna-splicing Factor Rse1
H	1	Cold Sensitive U2 Snrna Suppressor 1
I	1	Pre-mrna-splicing Factor Prp11
J	1	Pre-mrna-splicing Factor Rds3
K	1	Rds3 Complex Subunit 10
O	1	Pre-mrna-splicing Factor Prp46
P	1	Pre-mrna-processing Protein 45
Q	1	Pre-mrna-splicing Factor Slt11
R	1	Pre-mrna-splicing Factor Cwc2
S	1	Pre-mrna-splicing Factor Cwc15
T	1	Pre-mrna-splicing Factor Bud31
U	1	Pre-mrna Leakage Protein 1
V	1	U2 Snrnp Component Ist3
W	1	Pre-mrna-splicing Factor Cwc26
Y	1	Pre-mrna-splicing Factor Atp-dependent RNA Helicase-like Protein Prp2
Z	1	Pre-mrna-splicing Factor Cwc22
a	1	Pre-mrna-splicing Factor Cwc24
b	1	Peptidyl-prolyl Isomerase Cwc27
c	1	Pre-mrna-splicing Factor Cef1
G	1	U2 Snrnp Component Hsh155
d	1	Pre-mrna-splicing Factor Clf1
X	1	Pre-mrna-splicing Factor Cwc21
v	1	Pre-mrna-splicing Factor Syf1
e	1	Protein Hsh49
f	1	Pre-mrna-splicing Factor Syf2
o p q r	4	Pre-mrna-processing Factor 19
t	1	Pre-mrna-splicing Factor Snt309
n	1	Pre-mrna-processing Factor 17
k	1	Small Nuclear Ribonucleoprotein-associated Protein B
i	1	Small Nuclear Ribonucleoprotein E
h	1	Small Nuclear Ribonucleoprotein F
j	1	Small Nuclear Ribonucleoprotein G
l	1	Small Nuclear Ribonucleoprotein SM D3
m	1	Small Nuclear Ribonucleoprotein SM D1
g	1	Small Nuclear Ribonucleoprotein SM D2
Nucleotides(5 molecules)
D	1	Saccharomyces Cerevisiae Strain Cdrdr_sf_h Chromosome VII Sequence
E	1	Saccharomyces Cerevisiae Strain T.52_2h Chromosome XII Sequence
L	1	U2 Snrna
N	1	Pre-mrna
M	1	Pre-mrna
Chemicals and Non-standard biopolymers (20 molecules)
1	1	GUANOSINE-5'-TRIPHOSPHATE (Link to PubChem not yet available)
2	5	MAGNESIUM ION (Link to PubChem not yet available)
3	13	ZINC ION (Link to PubChem not yet available)
4	1	ADENOSINE-5'-DIPHOSPHATE (Link to PubChem not yet available)

* Click molecule labels to explore molecular sequence information.

Citing MMDB

Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303