National Center for
Biotechnology Information

8CBJ: Cryo-EM structure of Otu2-bound cytoplasmic pre-40S ribosome biogenesis complex

PDB ID:

MMDB ID:

230495

PDB Deposition Date:

2023/1/25

Updated in MMDB:

2023/05

Experimental Method:

electron microscopy

Resolution:

3.8 Å

Source Organism:

Saccharomyces cerevisiae W303▼

Taxonomy	Molecule
Saccharomyces cerevisiae W303	b, c, d, e, g, h, i, j, k, l, A, B, C, D, E, F, G, H, I, J, L, N, O, P, Q, S, T, U, V, W, X, Y, Z, a , y, K
Saccharomyces cerevisiae	M, R, 2

Similar Structures:

Biological Unit for 8CBJ: 39-meric; determined by author

Molecular Components in 8CBJ
Label	Count	Molecule
Proteins (38 molecules)
b	1	40S Ribosomal Protein S27-a
c	1	40S Ribosomal Protein S28-a
d	1	Rps29a Isoform 1
e	1	40S Ribosomal Protein S30-a
g	1	Guanine Nucleotide-binding Protein Subunit Beta-like Protein
h	1	Pre-rrna-processing Protein Pno1
i	1	Enp1 Isoform 1
j	1	Ltv1 Isoform 1
k	1	Tsr1 Isoform 1
l	1	Non-specific Serine/threonine Protein Kinase
A	1	40S Ribosomal Protein S0-a
B	1	40S Ribosomal Protein S1-a
C	1	40S Ribosomal Protein S2
D	1	40S Ribosomal Protein S3
E	1	40S Ribosomal Protein S4-b
F	1	40S Ribosomal Protein S5
G	1	40S Ribosomal Protein S6-b
H	1	40S Ribosomal Protein S7-a
I	1	40S Ribosomal Protein S8-a
J	1	40S Ribosomal Protein S9-a
L	1	40S Ribosomal Protein S11-a
M	1	40S Ribosomal Protein S12
N	1	40S Ribosomal Protein S13
O	1	40S Ribosomal Protein S14-a
P	1	Rps15 Isoform 1
Q	1	40S Ribosomal Protein S16-a
R	1	40S Ribosomal Protein S17-a
S	1	40S Ribosomal Protein S18-a
T	1	40S Ribosomal Protein S19-a
U	1	40S Ribosomal Protein S20
V	1	40S Ribosomal Protein S21-a
W	1	40S Ribosomal Protein S22-a
X	1	40S Ribosomal Protein S23-a
Y	1	40S Ribosomal Protein S24-a
Z	1	40S Ribosomal Protein S25-a
a	1	20s-pre-rrna D-site Endonuclease Nob1
y	1	rRNA Adenine N(6)-methyltransferase
K	1	OTU Domain-containing Protein 2
Nucleotide(1 molecule)
2	1	20S Pre-ribosomal RNA
Chemicals and Non-standard biopolymers (48 molecules)
1	2	ZINC ION
2	46	MAGNESIUM ION

* Click molecule labels to explore molecular sequence information.

Citing MMDB