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IDs: 3423761 [UID] 11170418 [GenBank]
The assembly aRhiBiv1.1 is based on 43x PacBio data and 41x coverage 10X Genomics Chromium data generated at the Wellcome Sanger Institute, BioNano data generated by BioNano Genomics, and 39x coverage Hi-C data generated at the Wellcome Sanger Institute ... using a library created by Arima Genomics. The assembly process included the following sequence of steps: initial PacBio assembly generation with Falcon-unzip, retained haplotig identification with Purge Haplotigs, 10X based scaffolding with scaff10x, BioNano hybrid-scaffolding, Hi-C based scaffolding with SALSA2, Arrow polishing, and two rounds of FreeBayes polishing. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. more
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