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IDs: 202191 [UID] 1183568 [GenBank] 1401498 [RefSeq]
The Bald Eagle, Haliaeetus leucocephalus, DNA blood sample was obtained from a male with a Duke Unversity ID of CR32, and a Carolina raptor center ID of 06602. Total sequence assembled coverage of Illumina instrument reads was 103X (250bp, ... 3kb and 8kb inserts) using a genome size estimate of 1.4Gb. The combined sequence reads were assembled using an unpublished assisted assembly method (Richa Agarwala), and SOAPdenovo v. 1.0.3 software (BGI). Contamination was removed by screening the assembly against TGI (WUSTL) assemblies, several bacterial genomes and Illumina adapter sequences. Potential contaminants were confirmed by blasting against the NCBI nr/nt sequences. This draft assembly was referred to as Haliaeetus leucocephalus 4.0.1. The assembly is made up of a total of 1023 scaffolds with an N50 scaffold length of almost 9.1Mb and an N50 contig length of over 105Kb. The assembled genome spans almost 1.27Gb when including gaps. DNA samples can be obtained from: Dr. Erich Jarvis Bryan Research Building, Room 401E Research Drive Durham, North Carolina, 27710 For questions regarding this Bald Eagle assembly please contact Dr. Wesley Warren, Washington University School of Medicine (wwarren@genome.wustl.edu). Downloads of the sequence data are available via the NCBI SRA database. Credits: DNA source - Dr. Erich Jarvis, Duke University Sequencing - The Genome Institute, Washington University School of Medicine, St Louis, MO. Sequence assembly - The Genome Institute, Washington University School of Medicine, St Louis, MO; and Richa Agarwala, National Center for Biotechnology Information Citation upon use of this assembly in a manuscript: It is requested that users of this Haliaeetus leucocephalus 4.0.1 sequence assembly acknowledge Dr. Wesley Warren and The Genome Institute, Washington University School of Medicine in any publications that result from use of this sequence assembly. Haliaeetus leucocephalus 4.0.1 assembly stats: *** Contiguity: Contig *** Total contig number: 31786 Total contig bases: 1159246443 bp Average contig length: 36470 bp Maximum contig length: 752931 bp N50 contig length: 105493 bp N50 contig number: 3144 *** Contiguity: Supercontig *** Total supercontig number: 1023 Average supercontig length: 1133183 bp Maximum supercontig length: 30275662 bp N50 supercontig length: 9066221 bp N50 supercontig number: 40 *** Scaffold Distribution*** Scaffolds > 1M: 172 Scaffold 250K--1M: 65 Scaffold 100K--250K: 31 Scaffold 10--100K: 128 Scaffold 5--10K: 80 Scaffold 2--5K: 162 Scaffold 0--2K: 385 more
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