Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Download
IDs: 583111 [UID] 2684078 [GenBank] 2721448 [RefSeq]
Poeciliia latipinna (Sailfin molly) Sequence Assembly Release Notes The Sailfin molly DNA for shotgun sequencing is derived from an adult female (Poecilia latipinna; fish id # Pla-1442-1.1) within the laboratory of Dr. Manfred Schartl at the Biocenter of the ... University of Wuerzburg, Germany. Total sequence genome input coverage on the Illumina HiSeq instrument was 34x (20x fragments, and 14x 3kb). The combined sequence reads were assembled into contigs by Richa Agarwala by aligning the reads to the Poecilia formosa draft assembly. The P. latipinna assembly was further improved with the external scaffolding tool SSPACE (Boetzer 2010), and with a custom gap filling tool similar to IMAGE (Tsai 2010). This 1.0 version has been cleaned of contaminating contigs, and contigs 200bp and less were removed. The assembly is made up of a total of 17,988 scaffolds (including single contig scaffolds) with an N50 scaffold length of 250Mb (N50 contig length was 33kb). The assembly spans 815Mb. This work was supported by NIH grant R24 RR032658-01 to Dr. Warren, Washington University School of Medicine. DNA Source Contact: Dr. Manfred Schartl, Physiological Chemistry I University of Wuerzburg Biozentrum, Am Hubland Wuerzburg 97074 Germany Poecillia latipinna 1.0 Sequence and Assembly Credits: DNA source - Dr. Manfred Schartl, Physiologische Chemie, Biozentrum, Am Hubland, Universitat Wuerzburg, Germany. Genome Sequence - McDonnell Genome Institute, Washington University School of Medicine, St Louis, MO. Sequence Assembly - Richa Agarwala, National Center for Biotechnology Information, NIH, Bethesda, MD, USA, McDonnell Genome Institute, Washington University School of Medicine, St Louis, MO. Assembly curation - Pat Minx, McDonnell Genome Institute, Washington University School of Medicine, St Louis, MO. Citation upon use of this assembly in a manuscript: It is requested that users of this Poecillia latipinna 1.0 sequence assembly acknowledge Wesley Warren and McDonnell Genome Institute, Washington University School of Medicine in any publications that result from use of this sequence assembly. Poecillia latipinna 1.0 assembly statistics: (scaffold = supercontig) *** Contiguity: Contig *** Total contig number: 54625 Total contig bases: 679780316 bp Average contig length: 12444 bp Maximum contig length: 267274 bp N50 contig length: 33278 bp N50 contig number: 5928 *** Contiguity: Supercontig *** Total supercontig number: 17988 Average supercontig length: 37791 bp Maximum supercontig length: 1730811 bp N50 supercontig length: 250516 bp N50 supercontig number: 831 *** Scaffold Distribution *** Scaffolds > 1M: 13 Scaffold 250K--1M: 818 Scaffold 100K--250K: 1232 Scaffold 10--100K: 3108 Scaffold 5--10K: 817 Scaffold 2--5K: 1196 Scaffold 0--2K: 10804 more
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on