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IDs: 10471411 [UID] 27798128 [GenBank] 27910168 [RefSeq]
The assembly fThuMac1.1 is based on 46x PacBio data, 46x 10X Genomics Chromium data, BioNano data and Arima Hi-C data generated at the Wellcome Sanger Institute. The assembly process included the following sequence of steps: initial PacBio assembly generation ... with Hifiasm, retained haplotig separation with purge_dups, short-read polishing using FreeBayes-called variants from 10X Genomics Chromium reads aligned with LongRanger, and Hi-C based scaffolding with SALSA2. The mitochondrial genome was assembled using MitoHifi. Finally, the assembly was analysed and manually improved using gEVAL. Finally, the assembly was analysed and manually improved using gEVAL. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. Chromosome 1 contains a large inversion between sister chromatids at approximately 2.7-9.5Mb. more
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