Table 2Specificity and potency of human telomerase inhibition in vitro by template-targeting peptide nucleic acid (PNA) and phosphorothioate DNA (PS-DNA)

inhibitorlength (nt)5'-sequence-3'IC50arefs
PS-DNA 113-TAGGGTTAGACAA200 nM 23
PNA 213-TAGGGTTAGACAA1–5 nM 23,44
PS-DNA 313 scrambled-TGTAAGGAACTAG450 nM 23
PNA 413 scrambled-TGTAAGGAACTAGN/Db 23
PNA 513 mismatch-TAGACTTAGGGAA1 μM 44
PS-DNA 615-GTTAGGGTTAGACAA0.5 nM (at 25°C) 43
PS-DNA 715 scrambled-TCAGACATATACTGC1.3 nM (at 25°C) 43
target RNA sequence -GUCAAUCCCAAUCUGUUU41- 5'   

a IC50 values determined with the telomere repeat-amplification protocol (TRAP) at 37°C (unless otherwise mentioned).

b N/D: not detectable. The italicized bases within the sequences represent mismatched nucleotides.

From: PNA and Oligonucleotide Inhibitors of Human Telomerase

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