1Q33,1QVJ,6DRJ


Conserved Protein Domain Family
NUDIX_ADPRase_Nudt9

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cd03670: NUDIX_ADPRase_Nudt9 
ADP-ribose pyrophosphatase
ADP-ribose pyrophosphatase (ADPRase;EC 3.6.1.13, also known as NUDIX (nucleoside diphosphate-linked moiety X)) motif 9/Nudt9, catalyzes the hydrolysis of ADP-ribose to AMP and ribose-5-P. Like other members of the NUDIX hydrolase superfamily of enzymes, it is thought to require a divalent cation, such as Mg2+, for its activity. It also contains a 23-residue NUDIX motif (GX5EX7REUXEEXGU, where U = I, L or V) which functions as a metal binding site/catalytic site. In addition to the NUDIX motif, there are additional conserved amino acid residues, distal from the signature sequence, that correlate with substrate specificity. In humans, there are four distinct ADPRase activities, three putative cytosolic (ADPRase-I, -II, and -Mn) and a single mitochondrial enzyme (ADPRase-m). ADPRase-m is also known as NUDT9. It can be distinugished from the cytosolic ADPRase by a N-terminal target sequence unique to mitochondrial ADPRase. NUDT9 functions as a monomer.
Statistics
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PSSM-Id: 467538
Aligned: 26 rows
Threshold Bit Score: 165.576
Created: 7-Feb-2006
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
 
active siteNUDIX motif
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1QVJ: Homo sapiens ADPRase NUDT9 binds ribose 5-phosphate and 2Mg2+, contacts at 4A
  • Structure:1Q33: Homo sapiens ADPRase NUDT9 binds glucose, a cryoprotectant molecule, contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                           # #                                          ##            
1Q33_A      91 NPAGRTGLVGRGLLGRWGPNHAADPIITRWKrdssgnkixhpvsgkhiLQFVAIKRkd-cgEWAIPGGXVdpg------e 163  human
1QVJ_A      91 NPAGRTGLVGRGLLGRWGPNHAADPIITRWKrdssgnkixhpvsgkhiLQFVAIKRkd-cgEWAIPGGXVdpg------e 163  human
6DRJ_A    1305 NPGGRTGIRGKGALNTLGPNHILHPIFTRWRdae-----------hkvLEFLAVWEda-ekRWALLGGPAq--------- 1363 Danio rerio
CAD01139  1272 NPMGRTGLRGRGSLSCFGPNHTLYPMVTRWRrnedgaic--rksikkmLEVLVVKLpl-seHWALPGGSRepg------e 1342 human
EDO42032    36 NPRGRTGFSGRGAFAHWGPNHTATFVITRLKlnpsdghi-veendqvvWEFLAVKEad-etSWRLPEGEMk--------- 104  starlet sea anemone
EDQ87245  1377 NPYGRTGLSGRGSLGRWGPNWAANCLITRWRrdvhgnlv--erdgrcvLEFLAIERqs-fgGWALPGGFRndaed--rrg 1451 Monosiga brevico...
CBY09828   288 NPIGRTGISGRGELPNWGPNHAVDPIITRWKkga-----------tgiLEWIAVQRdt--gEWAIPDNDRvv-------- 346  Oikopleura dioica
CBX25722    97 NPNGRTGVTGRGVLRRYGPNYLVQILISRGQs---------------vREYIVVEPenpdeLVSFPEDFVdsteftfipp 161  Trichinella spir...
EGD78999   855 NPKGPTGIKGRGFLGRWGVNLAVDAVLTRWKrnedgtel--vregkkmLEFVAIHRkk-dgVWAIPGRFVrqgpk---dt 928  Salpingoeca sp. ...
GAA53128  1836 NPMGRTGLLGKGLLPRWGPNHSIVICITRWSrdprtgnq-vtrsnrgvLQYIALERtk--rLCIPWYLTDhsnrc--eld 1910 Clonorchis sinensis
Feature 1                                                                                      
1Q33_A     164 kiSATLKREFGEEALnslqkts--------------------------------------aekreieEKLHKLFsq---- 201  human
1QVJ_A     164 kiSATLKREFGEEALnslqkts--------------------------------------aekreieEKLHKLFsq---- 201  human
6DRJ_A    1364 --PDEPLAQVLERILgkk-----------------------------------------------lnEKTKTLLk----- 1389 Danio rerio
CAD01139  1343 mlPRKLKRILRQEHW----------------------------------------------------PSFENLLk----- 1365 human
EDO42032   105 -vTDYIPEQIIEDLLslakesile----------------------------------lsngslnlhDTLQHLCetc--g 147  starlet sea anemone
EDQ87245  1452 alERLLKHEIYQQDKltq----------------------------------------------vevDNLEELFd----- 1480 Monosiga brevico...
CBY09828   347 -vSGACISRIMEEKVfgkqe-------------------------------------------keteELLKRLFsy---t 379  Oikopleura dioica
CBX25722   162 klYDLLLKELSKFYDprh-----------------------------------------------atYLLEKTFk----- 189  Trichinella spir...
EGD78999   929 dsEDQIKALLKNEALitlvteavdihqdkytddedaqqasindsisgaegqgddkevqgvelspqerEVMKQLRrc---- 1004 Salpingoeca sp. ...
GAA53128  1911 tcVPKVISSFITRRArat----------------------------------------------lseKRVDRLLkrldka 1944 Clonorchis sinensis
Feature 1               # # #                                                                  
1Q33_A     202 dHLVIYKGYVDDPRNTDNAWXETEAVNYHDetgei-----xdnlxLEAGDDAGKVKWVDIndk----------------- 259  human
1QVJ_A     202 dHLVIYKGYVDDPRNTDNAWXETEAVNYHDetgei-----xdnlxLEAGDDAGKVKWVDIndk----------------- 259  human
6DRJ_A    1390 aGEEVYKGYVDDSRNTDNAWVETSIITLHCdkntplm-adlnhmvESSLSSHQPLQWREVssd----------------- 1451 Danio rerio
CAD01139  1366 cGMEVYKGYMDDPRNTDNAWIETVAVSVHFqdqndve-lnrlnsnLHACDSGASIRWQVVdrr----------------- 1427 human
EDO42032   148 qRVKLYSGYVDDPRNTDNAWLETNVVHFHDefregl----grlhqRLPHDDSKSLTWMKLthq----------------- 206  starlet sea anemone
EDQ87245  1481 sAHTMRSVYSHDPRNTDNAWVEVSCANFHDetgelt----krlhfKENQEHVKRIQWLMAhsg----------------- 1539 Monosiga brevico...
CBY09828   380 dADVIHKGMVDDVRATDNAWMETIALHFHDedgi--------ldnLKLAKNSAVWAELRDq------------------- 432  Oikopleura dioica
CBX25722   190 hKVELYRGFVADNRNTDNAWIEALTIEYNDpkykg-----igkikLNPDTNPMRLKWQILdlkffvdsinkvlsksdmsr 264  Trichinella spir...
EGD78999  1005 kIKQFERIYSDDPRNTDNAWIESQCFNFHDesgri------klrlSSKAEVADKVAWLPVmsh----------------- 1061 Salpingoeca sp. ...
GAA53128  1945 eVTQIFKGYLDDQLNADSAWTETVVINIHEgnakgaymgddflklFGEPATGEQCKWMEVgqs----------------- 2007 Clonorchis sinensis
Feature 1                           #           
1Q33_A     260 ---------------lkLYASHSQFIKLVAEKR 277  human
1QVJ_A     260 ---------------lkLYASHSQFIKLVAEKR 277  human
6DRJ_A    1452 ---------------acRCSYQREALRQIAHHH 1469 Danio rerio
CAD01139  1428 ---------------ipLYANHKTLLQKAAAEF 1445 human
EDO42032   207 ---------------anISAYHLNLLQKVSQQN 224  starlet sea anemone
EDQ87245  1540 ---------------lnLFASHGDLVREACQIH 1557 Monosiga brevicollis MX1
CBY09828   433 ----------------dLFGSHRKLLMLIADKK 449  Oikopleura dioica
CBX25722   265 askivravrvffdnlkaGSPGHIYTGLRVILSL 297  Trichinella spiralis
EGD78999  1062 ---------------trFFASHRHLLHMVANEH 1079 Salpingoeca sp. ATCC50818
GAA53128  2008 ---------------snLRTSHNYILKSVAESK 2025 Clonorchis sinensis

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