1Z0K


Conserved Protein Domain Family
Rab4

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cd04113: Rab4 
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Rab GTPase family 4 (Rab4)
Rab4 subfamily. Rab4 has been implicated in numerous functions within the cell. It helps regulate endocytosis through the sorting, recycling, and degradation of early endosomes. Mammalian Rab4 is involved in the regulation of many surface proteins including G-protein-coupled receptors, transferrin receptor, integrins, and surfactant protein A. Experimental data implicate Rab4 in regulation of the recycling of internalized receptors back to the plasma membrane. It is also believed to influence receptor-mediated antigen processing in B-lymphocytes, in calcium-dependent exocytosis in platelets, in alpha-amylase secretion in pancreatic cells, and in insulin-induced translocation of Glut4 from internal vesicles to the cell surface. Rab4 is known to share effector proteins with Rab5 and Rab11. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
Statistics
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PSSM-Id: 206696
Aligned: 10 rows
Threshold Bit Score: 304.743
Created: 9-Aug-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:The active conformation of Rab is stabilized by interations between the gamma phosphate of GTP and two critically conserved residues, Thr in switch I and Gly in switch II
  • Structure:1Z0K: Human Rab4q67l binds GTP and Mg2+, defined using 3.5 A contacts

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                #######         #   #  #                         #                      
1Z0K_A         9 FKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINvggkYVKLQIWDTAGLERFRSVTRSYYRGAAGALLVY 88  human
XP_757882     17 LKFIIIGEAGTGKSCLLHHFIHNQFKEQSAHTIGVEFSSRLIKignkSVKLQLWDTAGQERFRSVTRSYYRGAAGALLVY 96  Ustilago maydis...
CAA72626       9 FKYLIIGNSGTGKSCVLHQFLENKFKQDTAHTIGIEFGSKVVKiqdrSIKLQVWDTAGQERFRSLTKNYYNGAACALLVY 88  Trichinella pse...
NP_995877      9 FKFLIIGSAGSGKSCLLHHFIESKFKDDSSHTIGVEFGSRIVNvggkSVKLQIWDTAGQERFRSVTRSYYRGAAGALLVY 88  fruit fly
AAH44974       9 FKFLVIGSAGTGKSCLLHQFIESKFKQDSNHTIGVEFGSRIVNvggkSVKLQIWDTAGQERFRSVTRSYYRGAAGALLVY 88  African clawed ...
NP_001004002   9 FKFLVIGSAGTGKSCLLHQFIENKFKQDSNHTIGVEFGSRVVNvggkTVKLQIWDTAGQERFRSVTRSYYRGAAGALLVY 88  zebrafish
XP_629589      8 VKFIIIGSQSVGKSCLLFRFIDNKFKSQSTHTIGVDFSSRVVDiqgkNVKLQIWDTAGQERFRSVVISYYRGSAGVALVY 87  Dictyostelium d...
AAW24755      11 FKFLIIGAAGSGKTCILRRYTEGKFIHNMPHTIGAEFGSKIINvdgtTVKIQIWDTAGQERFRSMARSYYHDAVGALLVY 90  Schistosoma jap...
AAW43912      16 FKFIVIGEAGTGKSCLLYQCIHEQFKENSSHTIGVEFSSKTLRigdkNIKLQLWDTAGQERFRSVTRSYYRGAAGAILVY 95  Cryptococcus ne...
Q9GP33        10 FKFLIIGNAGTGKTCILRRYTERKFFPNTQHTIGAEFGSRVISvdgtHVKIQIWDTAGQERFRSMARSYYHDAVGTLLVY 89  Echinococcus mu...
Feature 1                                        ## ##                           ##              
1Z0K_A        89 DITSRETYNALTNWLTDARMLASQNIVIILCGNKKDLda-dREVTFLEASRFAQENelMFLETSALTGEDVEEAFVQCAR 167 human
XP_757882     97 DITKRSTFEPLSRWLTDARALASPDLVVVLVGNKTDRgddeREVGYLEASKWANENgvLFLETSSITGENVEAPFALAAR 176 Ustilago maydis...
CAA72626      89 DITCRQSFNAIAQWLSDARSLASPQIIVILIGNKKDLed-rREVTFMEASQFAQENgmLFLETSALTGENIEETFLRCAR 167 Trichinella pse...
NP_995877     89 DATSRDSFNALTNWLNDARTLASPNIVILLVGNKKDLee-aRDVTFLEASTFAQENelIFLETSAKTGENVEEAFLKCSK 167 fruit fly
AAH44974      89 DIASRETYNALTNWLTDARTLASPNIIIILCGNKKDLda-dREVTFLEASRFAQENelMFLETSALTGENVEEAFLKCAR 167 African clawed ...
NP_001004002  89 DITSRETYNALTNWLTDARTLASPNIVIILCGNKKDLda-dREVTFLEASRFAQENelMFLETSALTGENVEEAFLKCAR 167 zebrafish
XP_629589     88 DVTNRESYNHITNWLSDVKSLASPDVTIILVGNKADLte-qREVTFLEASRIAQENglLFMETSALTGEGVEEMFLKCTR 166 Dictyostelium d...
AAW24755      91 DVTNRDSFNDLGYWLSDARTLASPDIMIVLVGNKKDLqdtdGQVTQWEASAFAQDNdlHFLETSALTGENVEEAFTECVR 170 Schistosoma jap...
AAW43912      96 DITSRQSFVNLSRWLTDCRALASPHLVVVLVGNKLDKee-dREVEYTEGSRWAQENglLFVEVSSLTGKNVSTPFLLAGR 174 Cryptococcus ne...
Q9GP33        90 DITNRTSFGAVEQWLGDARHLATPGVVVILVGNKKDLrdtdGQVTHWEANTFAQENglQFIETSALTGENIDDAFTSCVR 169 Echinococcus mu...
Feature 1          
1Z0K_A       168 KI 169 human
XP_757882    177 SI 178 Ustilago maydis 521
CAA72626     168 SI 169 Trichinella pseudospiralis
NP_995877    168 TI 169 fruit fly
AAH44974     168 TI 169 African clawed frog
NP_001004002 168 SI 169 zebrafish
XP_629589    167 TI 168 Dictyostelium discoideum AX4
AAW24755     171 SL 172 Schistosoma japonicum
AAW43912     175 TI 176 Cryptococcus neoformans var. neoformans JEC21
Q9GP33       170 VL 171 Echinococcus multilocularis

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