1W0G,3UA1,2J0D


Conserved Protein Domain Family
CYP3A

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cd20650: CYP3A 
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cytochrome P450 family 3, subfamily A
The cytochrome P450 3A (CYP3A) subfamily, the most abundant CYP subfamily in the liver, consists of drug-metabolizing enzymes. In humans, there are at least four isoforms: CYP3A4, 3A5, 3A7, and 3A3. CYP3A enzymes are embedded in the endoplasmic reticulum, where they can catalyze a wide variety of biochemical reactions including hydroxylation, N-demethylation, O-dealkylation, S-oxidation, deamination, or epoxidation of substrates. They oxidize a variety of structurally unrelated compounds including steroids, fatty acids, and xenobiotics. The CYP3A subfamily belongs to the large cytochrome P450 (P450, CYP) superfamily of heme-containing proteins that catalyze a variety of oxidative reactions of a large number of structurally different endogenous and exogenous compounds in organisms from all major domains of life. CYPs bind their diverse ligands in a buried, hydrophobic active site, which is accessed through a substrate access channel formed by two flexible helices and their connecting loop.
Statistics
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PSSM-Id: 410743
Aligned: 13 rows
Threshold Bit Score: 727.286
Created: 3-Oct-2017
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
heme bindingchemical
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:heme binding site [chemical binding site]
Evidence:
  • Structure:1W0G: Human CYP3A4 binds heme; contacts at 4A
  • Structure:3UA1: Human CYP3A4 binds heme; contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                              #              ##      #   #      #       
1W0G_A        45 KYGKVWGFYDGqqPVLAITdPDMIKTVLVKECysvFTNRRPFGPV--GFMKSAISIAeDEEWKRLRSLLSPTFTsgKLKE 122 human
P08684        67 KYGKVWGFYDGqqPVLAITdPDMIKTVLVKECysvFTNRRPFGPV--GFMKSAISIAeDEEWKRLRSLLSPTFTsgKLKE 144 human
3UA1_A        47 KYGKVWGFYDGqqPVLAITdPDMIKTVLVKECysvFTNRRPFGPV--GFMKSAISIAeDEEWKRLRSLLSPTFTsgKLKE 124 human
2J0D_A        45 KYGKVWGFYDGqqPVLAITdPDMIKTVLVKECysvFTNRRPFGPV--GFMKSAISIAeDEEWKRLRSLLSPTFTsgKLKE 122 human
NP_001001751  67 KYGKIWGIYDGrqPAVVVMdPQIIKTVLVKECystFTNRRRTDLA--GVLRNAVSLAeDDQWKRLRTVLSPTFTsgKLKE 144 chicken
AAH60496      67 KYGKTWGLYDGrqPVLAIMdPAIIKTILVKECytnFTNRRNFGLS--GPLESAITAAeDEQWKRIRSVLSPTFTsgKLKE 144 African clawed ...
AAH85438      57 KYGKVWGIYDGrlPILMVTdLEMIKTIMVKECystFTNRREVNVDlaGPLADGISAVkDERWKRIRGTLSPYFTsgRLKE 136 zebrafish
Q8AXY5        66 KYGKVWGIYDGrqPVLCVTdPEIIKAVLVKEClsfFTNRRNFHLN--GPLYDALSVAeDDQWKRIRSVLSPSFTsgRLKE 143 killifish
P04800        67 KYGKIWGLFDGqmPLFAITdTEMIKNVLVKECfsvFTNRRDFGPV--GIMGKAVSVAkDEEWKRYRALLSPTFTsgRLKE 144 Norway rat
P05183        67 KYGKIWGLFDGqtPVFAIMdTEMIKNVLVKECfsvFTNRRDFGPV--GIMGKAVSVAkDEEWKRYRALLSPTFTsgRLKE 144 Norway rat
P20815        67 KYGKMWGTYEGqlPVLAITdPDVIRTVLVKECysvFTNRRSLGPV--GFMKSAISLAeDEEWKRIRSLLSPTFTsgKLKE 144 human
P11707        65 KYGKMWGLFDGrqPLMVITdPDMIKTVLVKECysvFTNRRSFGPV--GFMKKAVSISeDEDWKRVRTLLSPTFTsgKLKE 142 rabbit
P24462        67 KYRKVWGIYDCqqPMLAITdPDMIKTVLVKECysvFTNRRPFGPV--GFMKNAISIAeDEEWKRIRSLLSPTFTsgKLKE 144 human
Feature 1                                                                                        
1W0G_A       123 MVPIIAQYGDVLVRNLRREaetgkpvtlkdVFGAYSMDVITSTSfgvnidsLNNPQDPFVENTKKLLRFDFLDPFFLSIT 202 human
P08684       145 MVPIIAQYGDVLVRNLRREaetgkpvtlkdVFGAYSMDVITSTSfgvnidsLNNPQDPFVENTKKLLRFDFLDPFFLSIT 224 human
3UA1_A       125 MVPIIAQYGDVLVRNLRREaetgkpvtlkdVFGAYSMDVITSTSfgvnidsLNNPQDPFVENTKKLLRFDFLDPFFLSIT 204 human
2J0D_A       123 MVPIIAQYGDVLVRNLRREaetgkpvtlkdVFGAYSMDVITSTSfgvnidsLNNPQDPFVENTKKLLRFDFLDPFFLSIT 202 human
NP_001001751 145 MFPTMKYFGEMLVKNIQKRveknssvpvkdFFGSYSMDVVTSTSfgvnidsMNNPKSPFVREMQKLTKFDFFDPVFILSF 224 chicken
AAH60496     145 MFQIMKDYSDILVKNIQDNvgkdepctmkdVIGAYSMDVITSTSfsvnidsLKNPSHPFVVHIKNLLKGGLLNPLIILVV 224 African clawed ...
AAH85438     137 IFPIAVTHADRFIKNMQKRdheqp-vkikeVVAPYSLDVVTSSSfsvdidsINNPDDPFVTNIKNFLKFSLFSPLILFLT 215 zebrafish
Q8AXY5       144 MFEIMKNHSANLIRSMKKKadkdepldlkeFFGSYSMDVVTSTAfsvdidsLNNPSDPFVTNIKKMLKFDFLNPLFLAVA 223 killifish
P04800       145 MFPIIEQYGDILVKYLKQEaetgkpvtmkkVFGAYSMDVITSTSfgvnvdsLNNPKDPFVEKTKKLLRFDFFDPLFLSVV 224 Norway rat
P05183       145 MFPIIEQYGDILVKYLKQEaetgkpvtmkkVFGAYSMDVITSTSfgvnvdsLNNPKDPFVEKTKKLLRFDFFDPLFLSVV 224 Norway rat
P20815       145 MFPIIAQYGDVLVRNLRREaekgkpvtlkdIFGAYSMDVITGTSfgvnidsLNNPQDPFVESTKKFLKFGFLDPLFLSII 224 human
P11707       143 MLPIIAQYGDVLVKNLRQEaekgkpvdlkeIFGAYSMDVITGTSfgvnidsLRNPQDPFVKNVRRLLKFSFFDPLLLSIT 222 rabbit
P24462       145 MVPIIAQYGDVLVRNLRREaetgkpvtlkhVFGAYSMDVITSTSfgvsidsLNNPQDPFVENTKKLLRFNPLDPFVLSIK 224 human
Feature 1                                                                                        
1W0G_A       203 VFPflipilevlNICVFPREVTNFLRKSVKRMKESRledtqkhRVDFLQLMIDSQnske-------teshKALSDLELVA 275 human
P08684       225 VFPflipilevlNICVFPREVTNFLRKSVKRMKESRledtqkhRVDFLQLMIDSQnske-------teshKALSDLELVA 297 human
3UA1_A       205 VFPflipilevlNICVFPREVTNFLRKSVKRMKESRledtqkhRVDFLQLMIDSQnske-------teshKALSDLELVA 277 human
2J0D_A       203 VFPflipilevlNICVFPREVTNFLRKSVKRMKESRledtqkhRVDFLQLMIDSQnske-------teshKALSDLELVA 275 human
NP_001001751 225 VCPfltplmakmNISFFPSDAVDFFMRSIDKIKKDRerethtgRVDFLQMMIESQksdsngs-sdakhsyKALSDIEVLS 303 chicken
AAH60496     225 VFPflrpifeglNINFFPKNFTQFFTNAVTSFREKRkkgdhtgRVDFLQLMVDSSvtegnd----sgnqhKALTDVEIMA 300 African clawed ...
AAH85438     216 LFPsianllgkmGISVFSRSIMNFFYTALRKIKDEHkds--ngRVDFLRLMIQNQipddqakdttseqpvKGLTDHEILS 293 zebrafish
Q8AXY5       224 FFPflgpilekfELSFFPKSVTDFFYASLEKIKSNReasqqksRVDFLQLMIDSQknsg-------aqqdKSLTDHEILS 296 killifish
P04800       225 LFPfltpiyemlNICMFPKDSIEFFKKFVYRMKETRldsvqkhRVDFLQLMMNAHndskd------keshTALSDMEITA 298 Norway rat
P05183       225 LFPfltpiyemlNICMFPKDSIAFFQKFVHRIKETRldskhkhRVDFLQLMLNAHnnskd------evshKALSDVEIIA 298 Norway rat
P20815       225 LFPfltpvfealNVSLFPKDTINFLSKSVNRMKKSRlndkqkhRLDFLQLMIDSQnske-------teshKALSDLELAA 297 human
P11707       223 LFPfltpifealHISMFPKDVMDFLKTSVEKIKDDRlkdkqkrRVDFLQLMINSQnske-------idshKALDDIEVVA 295 rabbit
P24462       225 VFPfltpilealNITVFPRKVISFLTKSVKQIKEGRlketqkhRVDFLQLMIDSQnskd-------sethKALSDLELMA 297 human
Feature 1            #  ##  #                                                            #  # #  
1W0G_A       276 QSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPnkapptydtvlqMEYLDMVVNETLRLFPIAMRLERVC 355 human
P08684       298 QSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPnkapptydtvlqMEYLDMVVNETLRLFPIAMRLERVC 377 human
3UA1_A       278 QSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPnkapptydtvlqMEYLDMVVNETLRLFPIAMRLERVC 357 human
2J0D_A       276 QSIIFIFAGYETTSSVLSFIMYELATHPDVQQKLQEEIDAVLPnkapptydtvlqMEYLDMVVNETLRLFPIAMRLERVC 355 human
NP_001001751 304 QAFIFIFAGYEPTSNTLGYLAYQLALHPDVQQKVVNEIDTILPnkapltyeaimqLEYLDMAVNETLRLYPLGGRLERTC 383 chicken
AAH60496     301 QSIIFIFAGYETTSTALSYLFYCLATHPDVQQRLHEEIDSFLPdkasptydilmqMEYLDMVIQETLRLYPPAGRLERVS 380 African clawed ...
AAH85438     294 QSFIFILGGYETTSTTLSFLLHNLATNPDCLEKLVEEIDKNFPldtpisydalmkMDYLEMSINESMRLLPTAPRLERVC 373 zebrafish
Q8AXY5       297 QSMIFIFAGYETSSSSLTFLAYNLATNPEVMKKLQEEIDATFPnkapvhyqplmeMEYLDCVINESLRLFPIAARLERVA 376 killifish
P04800       299 QSIIFIFAGYEPTSSTLSFVLHSLATHPDTQKKLQEEIDRALPnkapptydtvmeMEYLDMVLNETLRLYPIGNRLERVC 378 Norway rat
P05183       299 QSVIFIFAGYETTSSTLSFVLYFLATHPDIQKKLQEEIDGALPskapptydivmeMEYLDMVLNETLRLYPIGNRLERVC 378 Norway rat
P20815       298 QSIIFIFAGYETTSSVLSFTLYELATHPDVQQKLQKEIDAVLPnkapptydavvqMEYLDMVVNETLRLFPVAIRLERTC 377 human
P11707       296 QSIIILFAGYETTSSTLSFIMHLLATHPDVQQKLQEEIDTLLPnkelatydtlvkMEYLDMVVNETLRLYPIAGRLERVC 375 rabbit
P24462       298 QSIIFIFAGYETTSSVLSFIIYELATHPDVQQKVQKEIDTVLPnkapptydtvlqLEYLDMVVNETLRLFPVAMRLERVC 377 human
Feature 1                                                                ####  #####   #         
1W0G_A       356 KKDVEINgMFIPKGVVVMIPSYALHrDPKYWtEPEKFLPERFSKKNkdnidPYIYTPFGSGpRNCIGMRFALMNMKLALI 435 human
P08684       378 KKDVEINgMFIPKGVVVMIPSYALHrDPKYWtEPEKFLPERFSKKNkdnidPYIYTPFGSGpRNCIGMRFALMNMKLALI 457 human
3UA1_A       358 KKDVEINgMFIPKGVVVMIPSYALHrDPKYWtEPEKFLPERFSKKNkdnidPYIYTPFGSGpRNCIGMRFALMNMKLALI 437 human
2J0D_A       356 KKDVEINgMFIPKGVVVMIPSYALHrDPKYWtEPEKFLPERFSKKNkdnidPYIYTPFGSGpRNCIGMRFALMNMKLALI 435 human
NP_001001751 384 KRDVEINgVTIPKGTIVIIPPYTLHrSPEYWpNPEEFRPERFSKENkdnidPYTYLPFGAGpRNCIGMRFALLTLKVAIT 463 chicken
AAH60496     381 KQNIEINgVSIPKGTVTMIPAYVLQrDPDYWpEPEEFRPERFSKENrethtPFTFLPFGDGpRNCIGMRFALLSMKVAIV 460 African clawed ...
AAH85438     374 KKTVELNgITIPKDTLVAIPTYVLNrDPQLWdSPQEFRPERFSPENkseflQYAFMPFGLGpRNCIGMRFALMIVKLLVV 453 zebrafish
Q8AXY5       377 KAAVEINgIVIPKDMVVMIPTWPLHrDPEIWpEPEAFKPERFSKKNkdnidPYIYMPFGSGpRNCIGMRFALVLIKLAVV 456 killifish
P04800       379 KKDVEINgVFMPKGSVVMIPSYALHrDPQHWpEPEEFRPERFSKENkgsidPYVYLPFGNGpRNCIGMRFALMNMKLALT 458 Norway rat
P05183       379 KKDIELDgLFIPKGSVVTIPTYALHhDPQHWpKPEEFHPERFSKENkgsidPYVYLPFGNGpRNCIGMRFALMNMKLALT 458 Norway rat
P20815       378 KKDVEINgVFIPKGSMVVIPTYALHhDPKYWtEPEEFRPERFSKKKds-idPYIYTPFGTGpRNCIGMRFALMNMKLALI 456 human
P11707       376 KKDVDINgTFIPKGTIVMMPTYALHrDPQHWtEPDEFRPERFSKKNkdninPYIYHPFGAGpRNCLGMRFALMNIKLALV 455 rabbit
P24462       378 KKDVEINgMFIPKGVVVMIPSYVLHhDPKYWtEPEKFLPERFSKKNkdnidPYIYTPFGSGpRNCIGMRFALVNMKLALV 457 human
Feature 1                                           
1W0G_A       436 RVLQNFSFkpckETQIPLKLSlgGLLQPEKPVVLK 470 human
P08684       458 RVLQNFSFkpckETQIPLKLSlgGLLQPEKPVVLK 492 human
3UA1_A       438 RVLQNFSFkpckETQIPLKLSlgGLLQPEKPVVLK 472 human
2J0D_A       436 RVLQNFSFkpckETQIPLKLSlgGLLQPEKPVVLK 470 human
NP_001001751 464 AVLQHFTFqvckETQIPLKLQslGLTTPEKPIVLK 498 chicken
AAH60496     461 SMLQNFSVrpcaETLIPMEYStiGVLQPKKPIVLK 495 African clawed frog
AAH85438     454 KLLQNFSLetckETQIPLELT--PVFQPKVPITLK 486 zebrafish
Q8AXY5       457 EILQQYSFsvckETEVPFEMDiqGLLAPKRPIQLK 491 killifish
P04800       459 KVLQNFSFqpckETQIPLKLSrqGLLQPTKPIILK 493 Norway rat
P05183       459 KVLQNFSFqpckETQIPLKLSrqAILEPEKPIVLK 493 Norway rat
P20815       457 RVLQNFSFkpckETQIPLKLDtqGLLQPEKPIVLK 491 human
P11707       456 RLMQNFSFklckETQVPLKLGkqGLLQPEKPIVLK 490 rabbit
P24462       458 RVLQNFSFkpckETQIPLKLRfgGLLLTEKPIVLK 492 human

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