2CXJ,1YUT


Conserved Protein Domain Family
S-100A13

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cd05022: S-100A13 
Click on image for an interactive view with Cn3D
S-100A13: S-100A13 domain found in proteins similar to S100A13. S100A13 is a calcium-binding protein belonging to a large S100 vertebrate-specific protein family within the EF-hand superfamily of calcium-binding proteins. Note that the S-100 hierarchy, to which this S-100A13 group belongs, contains only S-100 EF-hand domains, other EF-hands have been modeled separately. S100A13 is involved in the cellular export of interleukin-1 (IL-1) and of fibroblast growth factor-1 (FGF-1), which plays an important role in angiogenesis and tissue regeneration. Export is based on the CuII-dependent formation of multiprotein complexes containing the S100A13 protein. Assembly of these complexes occurs near the inner surface of the plasma membrane. Binding of two Ca(II) ions per monomer triggers key conformational changes leading to the creation of two identical and symmetrical Cu(II)-binding sites on the surface of the protein, close to the interface between the two monomers. These Cu(II)-binding sites are unique among the S100 proteins, which are reported to bind Cu(II) or Zn(II) ions in addition to Ca(II) ions. In addition, the three-dimensional structure of S100A13 differs significantly from those of other S100 proteins; the hydrophobic pocket that largely contributes to protein-protein interactions in other S100 proteins is absent in S100A13. The structure of S100A13 contains a large patch of negatively charged residues flanked by dense cationic clusters, formed mostly from positively charged residues from the C-terminal end, which plays major role in binding FGF-1.
Statistics
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PSSM-Id: 240149
Aligned: 11 rows
Threshold Bit Score: 94.7193
Created: 29-Dec-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:Ca2+ binding site [ion binding site]
Evidence:
  • Structure:1YUT_A, human S100a13 bound with calcium ions, contacts at 3.5 A
  • Comment:the N-terminal EF hand is a S100 specific feature while the C-terminal canonical EF hand is similar to the EF hands in calmodulin, troponin C, and parvalbumin
  • Comment:the second binding site (C-terminal EF hand) has a greater affinity for Ca2+ than the first (N-terminal EF hand)

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         #     #  #    #                             # # # #    #       
2CXJ_A         7 TELEAAIETVVSTFFTFAgr-egRKGSLNINEFKELATQQLPHLLKdv---gSLDEKMKTLDVNQDSELRFSEYWRLIGE 82  house mouse
1YUT_A         7 TELEESIETVVTTFFTFArq-egRKDSLSVNEFKELVTQQLPHLLKdv---gSLDEKMKSLDVNQDSELKFNEYWRLIGE 82  human
Q9HCY8        16 SDVERAIETLIKNFHQYSve--gGKETLTPSELRDLVTQQLPHLMPsn---cGLEEKIANLGSCNDSKLEFRSFWELIGE 90  human
XP_855157     16 SEVERAIETLIRSFHQYSve--gGKETLTPSELQALVTQQLPHLMPsn---yGLEEKIANLGSCTDSKLEFGSFWELIGE 90  dog
XP_428126      7 TPVEMAIETIVSVFVSHAgk-egRMGTLTATAFQELLRLQLPNLMKdv---pSLEEQMRALDVSTEQELTFEEFWKLMGE 82  chicken
CAF95168       6 SELELAIHTLVSEFHKAAe----GGASMNTTQFQTMMSKQLPVVAKtqdneeGLGRLLQQMGVQNGQNVSFENFWVLINK 81  Tetraodon nigro...
CAK11183       6 SDLEKAINTLVTNFHAASk---dNQPTLKADEFKNLLSSQLPTLIKs----eQVGEVMKQMGVKDGEGISFKNFWGLIQN 78  zebrafish
XP_684054      6 TDLELAINTLVTNFYSASp---nNADSLTAQEFQSMLSKELPTMVKtagdqeGLSKILKDLNVEEGQGITFKDFWQLVES 82  zebrafish
Q96FQ6         6 TELEKAVIVLVENFYKYVskyslVKNKISKSSFREMLQKELNHMLSdtgnrkAADKLIQNLDANHDGRISFDEYWTLIGG 85  human
XP_001252240  16 RDVERTSETLTKDFHQHLve--gGKESLNPSKLRGLITQQLPHLMLsn---cGLEEKTANLGNCNNSKLEFGSFWELFGE 90  cattle
AAI28876      25 STMESAIKTVVGVFLKSSk----GKENLGGKEFQSLVKSQLKNILTgsedneAVKNMREQLDNNQDGKVSFQEYMSLVGY 100 zebrafish
Feature 1                      
2CXJ_A        83 LAKEVRKEKALGIR 96  house mouse
1YUT_A        83 LAKEIRKKKDLKIR 96  human
Q9HCY8        91 AAKSVKLERPVRGH 104 human
XP_855157     91 AARSVKLESPVRSS 104 dog
XP_428126     83 LVRALWREKEGRKK 96  chicken
CAF95168      82 QAVQVFGSTHKEKN 95  Tetraodon nigroviridis
CAK11183      79 LATTQHGLLSHEKL 92  zebrafish
XP_684054     83 LANNQFGLQSKEKQ 96  zebrafish
Q96FQ6        86 ITGPIAKLIHEQEQ 99  human
XP_001252240  91 AAKSVKLENTVQGS 104 cattle
AAI28876     101 LAQAVSEQRCLEAE 114 zebrafish

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