1PY1,3G2S,1UJJ


Conserved Protein Domain Family
VHS_GGA1

?
cd17009: VHS_GGA1 
Click on image for an interactive view with Cn3D
VHS (Vps27/Hrs/STAM) domain of ADP-ribosylation factor-binding protein GGA1
ADP-ribosylation factor-binding protein GGA1 (Golgi-localized, Gamma-ear-containing, Arf-binding 1) is also called Gamma-adaptin-related protein 1. It is expressed in human brain and affects the generation of amyloid beta-peptide, and may be involved in the pathogenesis of Alzheimer disease. It is a member of the GGA subfamily, which is comprised of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins involved in membrane trafficking between the Trans-Golgi Network (TGN) and endosomes. The VHS domain has a superhelical structure similar to the structure of the ARM (Armadillo) repeats and is present at the N-termini of proteins. GGA proteins have a multidomain structure consisting of an N-terminal VHS domain linked by a short proline-rich linker to a GAT (GGA and TOM) domain, which is followed by a long flexible linker to the C-terminal appendage, GAE (Gamma-Adaptin Ear) domain. The VHS domain of GGA proteins binds to the acidic-cluster dileucine (DxxLL) motif found on the cytoplasmic tails of cargo proteins trafficked between the Trans-Golgi Network and the endosomal system.
Statistics
?
PSSM-Id: 340806
Aligned: 8 rows
Threshold Bit Score: 286.495
Created: 26-May-2015
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:acidic-cluster-dileucine motif interaction site [polypeptide binding site]
Evidence:
  • Comment:Recognition site of acidic-cluster-dilleucine (DxxLL) signal
  • Structure:1PY1_D: VHS domain of human GGA1 bound to beta-secretase phosphoserine peptide; contacts at 3.5A
  • Structure:3G2S_A: VHS domain of human GGA1 binds Sorla C-terminal peptide; contacts at 4A

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                      ##
1PY1_D        10 TLEARINRATNPLNkeLDWASINGFCEQLNeDFEGPPLATRLLAHKIQSPQeWEAIQALTVLETCMKSCGKRFHDEVGKF 89  human
3G2S_A        11 TLEARINRATNPLNkeLDWASINGFCEQLNeDFEGPPLATRLLAHKIQSPQeWEAIQALTVLETCMKSCGKRFHDEVGKF 90  human
1UJJ_A         9 TLEARINRATNPLNkeLDWASINGFCEQLNeDFEGPPLATRLLAHKIQSPQeWEAIQALTVLETCMKSCGKRFHDEVGKF 88  human
AAH73000       5 SLEVQINRATNPLNkeQDWDGVQAFCDQLNqELEGPQLATRLLAHKIQSPQeWEAMQALTVLEACMKNCGKRFHSEVGKF 84  African clawed ...
AAH80229       9 SLESRINKATNPLNreTDWESIQLFCDQLSnEPEGPQLATRLLAHKIQSSQeWEAIQALMVLETCVKNCGKRFHNEVGKF 88  zebrafish
XP_007910141  28 SLESRLNKATNPLNrdLEWDYIQAFCDQLNkELEGPQLATRFLAHKIQSPQeWEAVQALTVLETCMKNCGKRFHSEVGKF 107 elephant shark
Q9UJY5         9 TLEARINRATNPLNkeLDWASINGFCEQLNeDFEGPPLATRLLAHKIQSPQeWEAIQALTVLETCMKSCGKRFHDEVGKF 88  human
XP_004949853   5 TLEARINKATNPLNkeLDWDGINAFCEQLNkELEGPPLATRLLAHKIQSPQeWEAIQALTVLESCMKSCGKRFHDEVGKF 84  chicken
Feature 1        #  #        ##                            #      #   #     
1PY1_D        90 RFLNELIKVVSPKYLGSRtSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLKKQGIVKS 148 human
3G2S_A        91 RFLNELIKVVSPKYLGSRtSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLKKQGIVKS 149 human
1UJJ_A        89 RFLNELIKVVSPKYLGSRtSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLKKQGIVKS 147 human
AAH73000      85 RFLNELIKVVSPKYLGSRsPEKVKLKILELLYSWTLGLPSEVKISEAYQMLKKQGIVKE 143 African clawed frog
AAH80229      89 RFLNELIKVVSPKYLGSRaPEPVKKKVLEMMYSWTVSLPEETKISDAYQMLKKQGIIKQ 147 zebrafish
XP_007910141 108 RFLNELIKVVSPKYLGTRaPEKVKTKVLELMFSWTVGLPDELKITEAYQMLKKQGILKQ 166 elephant shark
Q9UJY5        89 RFLNELIKVVSPKYLGSRtSEKVKNKILELLYSWTVGLPEEVKIAEAYQMLKKQGIVKS 147 human
XP_004949853  85 RFLNELIKVVSPKYLGSRtPEKVKSKILELMYSWTLGLPHEVKILEAYQMLKKQGIVKS 143 chicken

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap