mrck-1 Serine/threonine-protein kinase mrck-1;non-specific serine/threonine protein kinase [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: mrck-1
Official Full Name: Serine/threonine-protein kinase mrck-1;non-specific serine/threonine protein kinase
Primary source: WormBase:WBGene00006437
Locus tag: CELE_K08B12.5
See related: AllianceGenome:WB:WBGene00006437
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable protein serine/threonine kinase activity. Involved in several processes, including embryonic body morphogenesis; positive regulation of GTPase activity; and positive regulation of endocytic recycling. Located in cytoplasm. Is expressed in Ea and Ep. Used to study cerebral cavernous malformation. Orthologous to several human genes including CDC42BPA (CDC42 binding protein kinase alpha) and CDC42BPB (CDC42 binding protein kinase beta). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See mrck-1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 12

Sequence:: Chromosome: V; NC_003283.11 (6257392..6267287, complement)

Chromosome V - NC_003283.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003283.11

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MRCK-1 is required for activation of contractile actomyosin dynamics and elevated cortical tension in the apical cell cortex of endoderm precursor cells. MRCK-1 is apically localized by active Cdc42 at the external, cell-cell contact-free surfaces of apically constricting cells, downstream of cell fate determination mechanisms.
Title: MRCK-1 Drives Apical Constriction in C. elegans by Linking Developmental Patterning to Force Generation.
Data show that combined loss of ROCK and PAK, or ROCK and MRCK, completely prevented embryonic elongation, but a constitutively active form of MLC-4 could only rescue a lack of MRCK.
Title: Myosin II regulation during C. elegans embryonic elongation: LET-502/ROCK, MRCK-1 and PAK-1, three kinases with different roles.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

STS5-12 (e-PCR)
- UniSTS:242278

K08B12.1 (e-PCR)
- UniSTS:311469

K08B12.5 (e-PCR)
- UniSTS:311471

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actomyosin structure organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in embryo development ending in birth or egg hatching	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic body morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryonic morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in epithelial tube morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nematode larval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of endocytic recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of Golgi organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Serine/threonine-protein kinase mrck-1;non-specific serine/threonine protein kinase

NP_001300141.1

Confirmed by transcript evidence

NP_001300142.1

Confirmed by transcript evidence

NP_001300143.1

Confirmed by transcript evidence

NP_001300144.1

Confirmed by transcript evidence

NP_504599.2

Partially confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003283.11 Reference assembly

Range

6257392..6267287 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001313212.2 → NP_001300141.1 non-specific serine/threonine protein kinase [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

A0A0K3AUZ7

Conserved Domains (6) summary

smart00285
Location:1542 → 1580

PBD; P21-Rho-binding domain

smart00036
Location:1180 → 1492

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cd00029
Location:956 → 1005

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01243
Location:1014 → 1157

PH_MRCK; MRCK (myotonic dystrophy-related Cdc42-binding kinase) pleckstrin homology (PH) domain

cd05597
Location:81 → 415

STKc_DMPK_like; Catalytic domain of Myotonic Dystrophy protein kinase (DMPK)-like Serine/Threonine Kinases

TIGR02168
Location:452 → 756

SMC_prok_B; chromosome segregation protein SMC, common bacterial type
NM_072198.8 → NP_504599.2 Serine/threonine-protein kinase mrck-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_504599.2

Status: REVIEWED

UniProtKB/Swiss-Prot

O01583

Conserved Domains (7) summary

smart00285
Location:1544 → 1582

PBD; P21-Rho-binding domain

smart00036
Location:1182 → 1494

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

smart00220
Location:83 → 351

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1340
Location:494 → 786

COG1340; Uncharacterized coiled-coil protein, contains DUF342 domain [Function unknown]

cd00029
Location:958 → 1007

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01243
Location:1016 → 1159

PH_MRCK; MRCK (myotonic dystrophy-related Cdc42-binding kinase) pleckstrin homology (PH) domain

cd05597
Location:81 → 417

STKc_DMPK_like; Catalytic domain of Myotonic Dystrophy protein kinase (DMPK)-like Serine/Threonine Kinases
NM_001313213.3 → NP_001300142.1 non-specific serine/threonine protein kinase [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

A0A0K3AS73

Conserved Domains (10) summary

pfam08826
Location:530 → 583

DMPK_coil; DMPK coiled coil domain like

smart00285
Location:1262 → 1300

PBD; P21-Rho-binding domain

smart00036
Location:900 → 1212

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

COG1340
Location:212 → 504

COG1340; Uncharacterized coiled-coil protein, contains DUF342 domain [Function unknown]

cd00029
Location:676 → 725

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01243
Location:734 → 877

PH_MRCK; MRCK (myotonic dystrophy-related Cdc42-binding kinase) pleckstrin homology (PH) domain

pfam00169
Location:747 → 871

PH; PH domain

pfam15035
Location:366 → 492

Rootletin; Ciliary rootlet component, centrosome cohesion

pfam17075
Location:206 → 336

RRT14; Regular of rDNA transcription protein 14

cl21453
Location:1 → 135

PKc_like; Protein Kinases, catalytic domain
NM_001313214.3 → NP_001300143.1 non-specific serine/threonine protein kinase [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

A0A0K3AVQ9

Conserved Domains (10) summary

pfam08826
Location:532 → 585

DMPK_coil; DMPK coiled coil domain like

smart00285
Location:1264 → 1302

PBD; P21-Rho-binding domain

smart00036
Location:902 → 1214

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

COG1340
Location:214 → 506

COG1340; Uncharacterized coiled-coil protein, contains DUF342 domain [Function unknown]

cd00029
Location:678 → 727

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01243
Location:736 → 879

PH_MRCK; MRCK (myotonic dystrophy-related Cdc42-binding kinase) pleckstrin homology (PH) domain

pfam00169
Location:749 → 873

PH; PH domain

pfam15035
Location:368 → 494

Rootletin; Ciliary rootlet component, centrosome cohesion

pfam17075
Location:208 → 338

RRT14; Regular of rDNA transcription protein 14

cl21453
Location:1 → 137

PKc_like; Protein Kinases, catalytic domain
NM_001313215.3 → NP_001300144.1 non-specific serine/threonine protein kinase [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

A0A0K3AY02

Conserved Domains (8) summary

pfam08826
Location:133 → 186

DMPK_coil; DMPK coiled coil domain like

smart00285
Location:865 → 903

PBD; P21-Rho-binding domain

smart00036
Location:503 → 815

CNH; Domain found in NIK1-like kinases, mouse citron and yeast ROM1, ROM2

cd00029
Location:279 → 328

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01243
Location:337 → 480

PH_MRCK; MRCK (myotonic dystrophy-related Cdc42-binding kinase) pleckstrin homology (PH) domain

pfam00169
Location:350 → 474

PH; PH domain

pfam12709
Location:13 → 57

Kinetocho_Slk19; Central kinetochore-associated

pfam15035
Location:5 → 95

Rootletin; Ciliary rootlet component, centrosome cohesion