U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

NSL1 NSL1 component of MIS12 kinetochore complex [ Homo sapiens (human) ]

Gene ID: 25936, updated on 2-Nov-2024

Summary

Go to the top of the page Help
Official Symbol
NSL1provided by HGNC
Official Full Name
NSL1 component of MIS12 kinetochore complexprovided by HGNC
Primary source
HGNC:HGNC:24548
See related
Ensembl:ENSG00000117697 MIM:609174; AllianceGenome:HGNC:24548
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
DC8; MIS14; C1orf48
Summary
This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. There is a pseudogene of the 3' UTR region of this gene on chromosome X. [provided by RefSeq, Jul 2014]
Expression
Ubiquitous expression in brain (RPKM 2.0), lymph node (RPKM 1.8) and 25 other tissues See more
Orthologs
mouse all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See NSL1 in Genome Data Viewer
Location:
1q32.3
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (212726153..212791777, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (211971114..212036744, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (212899495..212965119, complement)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene long intergenic non-protein coding RNA 2773 Neighboring gene NANOG-H3K4me1 hESC enhancer GRCh37_chr1:212828983-212829800 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2512 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:212831435-212832251 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1802 Neighboring gene RFK pseudogene 5 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1803 Neighboring gene Sharpr-MPRA regulatory region 1202 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2513 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2514 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2515 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr1:212856142-212857341 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:212857741-212858264 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:212860079-212860578 Neighboring gene U6 spliceosomal RNA Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1804 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1805 Neighboring gene basic leucine zipper ATF-like transcription factor 3 Neighboring gene NANOG hESC enhancer GRCh37_chr1:212885887-212886440 Neighboring gene MPRA-validated peak687 silencer Neighboring gene MPRA-validated peak688 silencer Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:212932746-212933595 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:212933596-212934444 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:212963792-212964570 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2517 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:212967459-212967959 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2519 Neighboring gene TatD DNase domain containing 3 Neighboring gene RPS5 pseudogene 4

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. CENP-T provides a structural platform for outer kinetochore assembly. Nishino T, et al. EMBO J, 2013 Feb 6. PMID 23334297, Free PMC Article
  2. Hsp90-Sgt1 and Skp1 target human Mis12 complexes to ensure efficient formation of kinetochore-microtubule binding sites. Davies AE, et al. J Cell Biol, 2010 Apr 19. PMID 20404110, Free PMC Article
  3. Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores. Petrovic A, et al. Cell, 2016 Nov 3. PMID 27881301, Free PMC Article
  4. Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1. Kiyomitsu T, et al. Dev Cell, 2007 Nov. PMID 17981135
  5. The MIS12 complex is a protein interaction hub for outer kinetochore assembly. Petrovic A, et al. J Cell Biol, 2010 Sep 6. PMID 20819937, Free PMC Article

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
    Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
  2. The hMis14, which contains a tripartite-binding domain for HP1 and two other kinetochore proteins, hMis13 and blinkin, is a cornerstone for the assembly of the inner centromere and kinetochore.
    Title: Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes.
Submit: New GeneRIF Correction

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • DKFZp566O1646

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in attachment of spindle microtubules to kinetochore NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in cell division IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic sister chromatid segregation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of MIS12/MIND type complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of MIS12/MIND type complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of MIS12/MIND type complex NAS
Non-traceable Author Statement
more info
 PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
  
located_in kinetochore IDA
Inferred from Direct Assay
more info
 PubMed 
located_in kinetochore NAS
Non-traceable Author Statement
more info
 PubMed 
located_in nuclear speck IDA
Inferred from Direct Assay
more info
  
located_in nucleus NAS
Non-traceable Author Statement
more info
 PubMed 
part_of outer kinetochore IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle pole NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
kinetochore-associated protein NSL1 homolog
Names
NSL1, MIND kinetochore complex component, homolog
NSL1, MIS12 kinetochore complex component

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001042549.2 → NP_001036014.1  kinetochore-associated protein NSL1 homolog isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) contains an alternate exon, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.
    Source sequence(s)
    AA806390, AL591647, BU570983, DB045932
    Consensus CDS
    CCDS53474.1
    UniProtKB/Swiss-Prot
    Q96IY1
    Related
    ENSP00000486783.1, ENST00000626725.1
    Conserved Domains (1) summary
    pfam08641
    Location:82 → 166
    Mis14; Kinetochore protein Mis14 like

  2. NM_001297736.2 → NP_001284665.1  kinetochore-associated protein NSL1 homolog isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks two alternate exons, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.
    Source sequence(s)
    AA806390, AL591647, BE669670, DB045932
    UniProtKB/TrEMBL
    B4E071
    Conserved Domains (1) summary
    pfam08641
    Location:82 → 150
    Mis14; Kinetochore protein Mis14 like

  3. NM_001297737.2 → NP_001284666.1  kinetochore-associated protein NSL1 homolog isoform 4

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) lacks an alternate exon, which results in a frameshift, compared to variant 1. The encoded isoform (4) has a distinct C-terminus and is shorter than isoform 1.
    Source sequence(s)
    AA806390, AL591647, CK003923, DB045932
    Consensus CDS
    CCDS73025.1
    UniProtKB/TrEMBL
    Q5SY74
    Related
    ENSP00000355943.1, ENST00000366976.3
    Conserved Domains (1) summary
    pfam08641
    Location:82 → 166
    Mis14; Kinetochore protein Mis14 like

  4. NM_001297739.2 → NP_001284668.1  kinetochore-associated protein NSL1 homolog isoform 5

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) lacks a large segment of the 3' coding region and 3' UTR, which results in a frameshift, compared to variant 1. The encoded isoform (4) has a distinct C-terminus and is shorter than isoform 1.
    Source sequence(s)
    AA806390, AI703127, AL591647, DB045932
    Conserved Domains (1) summary
    pfam08641
    Location:82 → 170
    Mis14; Kinetochore protein Mis14 like

  5. NM_015471.4 → NP_056286.3  kinetochore-associated protein NSL1 homolog isoform 1

    See identical proteins and their annotated locations for NP_056286.3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AA806390, AK223260, AL591647
    Consensus CDS
    CCDS1509.1
    UniProtKB/Swiss-Prot
    E7ETD5, Q5SY75, Q96IY1, Q9H2M5, Q9NRN8, Q9Y415
    UniProtKB/TrEMBL
    B2R9C5, D3DTA2, Q53FM2
    Related
    ENSP00000355944.3, ENST00000366977.8
    Conserved Domains (1) summary
    pfam08641
    Location:82 → 196
    Mis14; Kinetochore protein Mis14 like

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

    Range
    212726153..212791777 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060925.1 Alternate T2T-CHM13v2.0

    Range
    211971114..212036744 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q96IY1.3 GenPept UniProtKB/Swiss-Prot:Q96IY1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous